miRNA: 3'- -GCGGag--UugCAU--UAC-AGCCACCGCGC- -5'
miRNA: 3'- gCGG-AGuuGCAUUA-CAGCCA-CCGcGC- -5'
miRNA: 3'- gCGG-AGUUGCA-------------UUACAGCCacCGCGc -5'
miRNA: 3'- -GCGgAGUUGCAUua--CAGCCa---CCGcgc -5'
Results 41 - 44 of 44 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 19068 | 3' | -54.1 | NC_004684.1 | + | 62957 | 0.71 | 0.554701 |
|
Target: 5'- gCGCCgacgAugGUGgcGUGcUCGGUGGUGCGc -3' miRNA: 3'- -GCGGag--UugCAU--UAC-AGCCACCGCGC- -5' |
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| 19068 | 3' | -54.1 | NC_004684.1 | + | 63546 | 0.66 | 0.8513 |
|
Target: 5'- uGCCuUCggUGUGGUcGUCGGUcGGCaCGc -3' miRNA: 3'- gCGG-AGuuGCAUUA-CAGCCA-CCGcGC- -5' |
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| 19068 | 3' | -54.1 | NC_004684.1 | + | 64636 | 0.67 | 0.76695 |
|
Target: 5'- uGCCgUCGGCGUcguggcgcaccgcGAUGUCGGc-GCGCa -3' miRNA: 3'- gCGG-AGUUGCA-------------UUACAGCCacCGCGc -5' |
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| 19068 | 3' | -54.1 | NC_004684.1 | + | 64799 | 0.66 | 0.825209 |
|
Target: 5'- cCGCgUCGGCGUAcucgGUCGGgucgGGCa-- -3' miRNA: 3'- -GCGgAGUUGCAUua--CAGCCa---CCGcgc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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