miRNA: 3'- -GCGGAGUUGCauuacaGCCACCGcGC- -5'
miRNA: 3'- -GCGGAG-UUGCAUUACAGC---CA----CCGCgC- -5'
miRNA: 3'- gCGGAGUUGCauua-CAGCCAcCGcGC- -5'
miRNA: 3'- -GCGGAGUUGCAUUACAgCCA--------CCGCgC- -5'
Results 41 - 44 of 44 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 19068 | 3' | -54.1 | NC_004684.1 | + | 4791 | 0.66 | 0.842816 |
|
Target: 5'- gCGCCUCGGCG------CGGUGGC-CGa -3' miRNA: 3'- -GCGGAGUUGCauuacaGCCACCGcGC- -5' |
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| 19068 | 3' | -54.1 | NC_004684.1 | + | 2190 | 0.68 | 0.747663 |
|
Target: 5'- gCGUCUCuGACGUAAUGUCGuucGUguacGGCGgGa -3' miRNA: 3'- -GCGGAG-UUGCAUUACAGC---CA----CCGCgC- -5' |
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| 19068 | 3' | -54.1 | NC_004684.1 | + | 1229 | 0.68 | 0.7058 |
|
Target: 5'- gGCCUCGGCGgccugGUCGGUcGCcCGg -3' miRNA: 3'- gCGGAGUUGCauua-CAGCCAcCGcGC- -5' |
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| 19068 | 3' | -54.1 | NC_004684.1 | + | 100 | 0.68 | 0.716398 |
|
Target: 5'- cCGUUUCGACGUAAUGU-GGUcuuuguacGGCGgGa -3' miRNA: 3'- -GCGGAGUUGCAUUACAgCCA--------CCGCgC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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