Results 41 - 44 of 44 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. |
strand![]() |
Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
19068 | 3' | -54.1 | NC_004684.1 | + | 64636 | 0.67 | 0.76695 |
Target: 5'- uGCCgUCGGCGUcguggcgcaccgcGAUGUCGGc-GCGCa -3' miRNA: 3'- gCGG-AGUUGCA-------------UUACAGCCacCGCGc -5' |
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19068 | 3' | -54.1 | NC_004684.1 | + | 22428 | 0.68 | 0.75787 |
Target: 5'- gGCCUCAACGccaucgccGUCGGcgcGGCgGCGa -3' miRNA: 3'- gCGGAGUUGCauua----CAGCCa--CCG-CGC- -5' |
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19068 | 3' | -54.1 | NC_004684.1 | + | 8401 | 0.68 | 0.726916 |
Target: 5'- gGCCUCGACGgcAUGUucCGuGUGuggaacccGCGCGc -3' miRNA: 3'- gCGGAGUUGCauUACA--GC-CAC--------CGCGC- -5' |
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19068 | 3' | -54.1 | NC_004684.1 | + | 33471 | 0.77 | 0.269691 |
Target: 5'- uGCCUCGGCGgcGgcugCGGUGGaCGCGg -3' miRNA: 3'- gCGGAGUUGCauUaca-GCCACC-GCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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