Results 1 - 17 of 17 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
19069 | 5' | -53.2 | NC_004684.1 | + | 46557 | 0.66 | 0.813571 |
Target: 5'- aCGcCGGUGCGCUUGGUGCcggAUUCccaGUCGc -3' miRNA: 3'- -GC-GCUACGCGAGUCGCG---UAAG---UAGUu -5' |
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19069 | 5' | -53.2 | NC_004684.1 | + | 23207 | 0.66 | 0.812615 |
Target: 5'- cCGCGAgGUGCU-GGCGCgcgccggGUUCAUCGc -3' miRNA: 3'- -GCGCUaCGCGAgUCGCG-------UAAGUAGUu -5' |
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19069 | 5' | -53.2 | NC_004684.1 | + | 42511 | 0.66 | 0.802947 |
Target: 5'- aCGCGAUcaacggcGCGCUCAcCGCcgUgGUCGg -3' miRNA: 3'- -GCGCUA-------CGCGAGUcGCGuaAgUAGUu -5' |
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19069 | 5' | -53.2 | NC_004684.1 | + | 37267 | 0.67 | 0.784074 |
Target: 5'- gCGCGGUGCcuguaGCUC-GCGCAggCGUUg- -3' miRNA: 3'- -GCGCUACG-----CGAGuCGCGUaaGUAGuu -5' |
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19069 | 5' | -53.2 | NC_004684.1 | + | 25496 | 0.67 | 0.773898 |
Target: 5'- uCGCGGUcgGC-CAGCGCGUUCAUg-- -3' miRNA: 3'- -GCGCUAcgCGaGUCGCGUAAGUAguu -5' |
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19069 | 5' | -53.2 | NC_004684.1 | + | 2140 | 0.67 | 0.763569 |
Target: 5'- uGCG-UGCGUUCGGCGCGUcgGUgAAc -3' miRNA: 3'- gCGCuACGCGAGUCGCGUAagUAgUU- -5' |
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19069 | 5' | -53.2 | NC_004684.1 | + | 23790 | 0.67 | 0.753101 |
Target: 5'- gCGCGAUGa--UCGGCGCuggCGUCAc -3' miRNA: 3'- -GCGCUACgcgAGUCGCGuaaGUAGUu -5' |
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19069 | 5' | -53.2 | NC_004684.1 | + | 35360 | 0.67 | 0.753101 |
Target: 5'- aCGCGGUGCcaGCUguGgGCGUUCccgCAAc -3' miRNA: 3'- -GCGCUACG--CGAguCgCGUAAGua-GUU- -5' |
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19069 | 5' | -53.2 | NC_004684.1 | + | 57070 | 0.68 | 0.742504 |
Target: 5'- gGCGGccUGCGUUgCGGCGCGcaCAUCGGu -3' miRNA: 3'- gCGCU--ACGCGA-GUCGCGUaaGUAGUU- -5' |
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19069 | 5' | -53.2 | NC_004684.1 | + | 52450 | 0.68 | 0.742504 |
Target: 5'- gGCucgGCGCagUCAGCGCAcacUCGUCAAa -3' miRNA: 3'- gCGcuaCGCG--AGUCGCGUa--AGUAGUU- -5' |
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19069 | 5' | -53.2 | NC_004684.1 | + | 55037 | 0.68 | 0.720975 |
Target: 5'- gGUGGUGCGCagugugccCAGCGCggUgAUCAAg -3' miRNA: 3'- gCGCUACGCGa-------GUCGCGuaAgUAGUU- -5' |
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19069 | 5' | -53.2 | NC_004684.1 | + | 8136 | 0.68 | 0.688025 |
Target: 5'- cCGCGucgGUGCUCgGGUGCAgcagaUCGUCAAc -3' miRNA: 3'- -GCGCua-CGCGAG-UCGCGUa----AGUAGUU- -5' |
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19069 | 5' | -53.2 | NC_004684.1 | + | 66251 | 0.68 | 0.688025 |
Target: 5'- -aCGAUcagcGUGCUCAGCGCGgcgUCGUCc- -3' miRNA: 3'- gcGCUA----CGCGAGUCGCGUa--AGUAGuu -5' |
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19069 | 5' | -53.2 | NC_004684.1 | + | 60878 | 0.71 | 0.5432 |
Target: 5'- gGCGAUGCGCaccuccUCGGCcuccaGUUCAUCGAc -3' miRNA: 3'- gCGCUACGCG------AGUCGcg---UAAGUAGUU- -5' |
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19069 | 5' | -53.2 | NC_004684.1 | + | 65810 | 0.71 | 0.519369 |
Target: 5'- aCGCug-GCGCUCAGCGCAgacaggcgcacCAUCAGc -3' miRNA: 3'- -GCGcuaCGCGAGUCGCGUaa---------GUAGUU- -5' |
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19069 | 5' | -53.2 | NC_004684.1 | + | 26343 | 0.83 | 0.105263 |
Target: 5'- aCGCGGUGCGCUCGGC-CAUUcCGUCGGg -3' miRNA: 3'- -GCGCUACGCGAGUCGcGUAA-GUAGUU- -5' |
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19069 | 5' | -53.2 | NC_004684.1 | + | 19860 | 1.07 | 0.00238 |
Target: 5'- gCGCGAUGCGCUCAGCGCAUUCAUCAAg -3' miRNA: 3'- -GCGCUACGCGAGUCGCGUAAGUAGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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