Results 61 - 68 of 68 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 19098 | 3' | -53.3 | NC_004684.1 | + | 55483 | 0.66 | 0.858983 |
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Target: 5'- nGGCUgucGGUGguGCCGCGCAcguuGAaUCCCGg -3' miRNA: 3'- -CUGA---CCAU--UGGCGCGUuuu-CU-GGGGC- -5' |
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| 19098 | 3' | -53.3 | NC_004684.1 | + | 33764 | 0.66 | 0.866332 |
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Target: 5'- uGACgaGGUAGCCgcccgacgauccgGCGCugccuGGCCCCa -3' miRNA: 3'- -CUGa-CCAUUGG-------------CGCGuuuu-CUGGGGc -5' |
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| 19098 | 3' | -53.3 | NC_004684.1 | + | 20062 | 0.66 | 0.867137 |
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Target: 5'- cGCguucGUGGCCGCGCucAAGcuggcguucaACCCCGa -3' miRNA: 3'- cUGac--CAUUGGCGCGuuUUC----------UGGGGC- -5' |
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| 19098 | 3' | -53.3 | NC_004684.1 | + | 11775 | 0.66 | 0.867137 |
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Target: 5'- gGugUGGUGGCCcCGUggGccgaccAGGCCaCCGu -3' miRNA: 3'- -CugACCAUUGGcGCGuuU------UCUGG-GGC- -5' |
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| 19098 | 3' | -53.3 | NC_004684.1 | + | 6835 | 0.66 | 0.867137 |
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Target: 5'- aGCUGGccuACgGCGCGAAGGGCgacgCCGu -3' miRNA: 3'- cUGACCau-UGgCGCGUUUUCUGg---GGC- -5' |
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| 19098 | 3' | -53.3 | NC_004684.1 | + | 41996 | 0.66 | 0.867137 |
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Target: 5'- ---cGGUGGCCGCugGCGAcaaccuccuGGAUCCCGu -3' miRNA: 3'- cugaCCAUUGGCG--CGUUu--------UCUGGGGC- -5' |
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| 19098 | 3' | -53.3 | NC_004684.1 | + | 49648 | 0.66 | 0.867137 |
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Target: 5'- aGGC-GGuUGGCCGCGCc--GGAUUCCGg -3' miRNA: 3'- -CUGaCC-AUUGGCGCGuuuUCUGGGGC- -5' |
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| 19098 | 3' | -53.3 | NC_004684.1 | + | 13684 | 0.66 | 0.875046 |
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Target: 5'- gGACaUGGUGGCCGguucgggcaCGCcGAAGAucaUCCCGa -3' miRNA: 3'- -CUG-ACCAUUGGC---------GCGuUUUCU---GGGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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