Results 61 - 68 of 68 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 19098 | 3' | -53.3 | NC_004684.1 | + | 57599 | 0.69 | 0.720962 |
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Target: 5'- cGACggUGGUGACCGUGCAcGAGcgguacggccagccACCCgGu -3' miRNA: 3'- -CUG--ACCAUUGGCGCGUuUUC--------------UGGGgC- -5' |
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| 19098 | 3' | -53.3 | NC_004684.1 | + | 57893 | 0.73 | 0.476228 |
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Target: 5'- uGGCUGGUGGCCGgGCuGAAgguggccgggcuGACCgCCGa -3' miRNA: 3'- -CUGACCAUUGGCgCGuUUU------------CUGG-GGC- -5' |
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| 19098 | 3' | -53.3 | NC_004684.1 | + | 58354 | 0.67 | 0.812944 |
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Target: 5'- uGCUGGcguuGCCGCGCcgguugccGCCCCGc -3' miRNA: 3'- cUGACCau--UGGCGCGuuuuc---UGGGGC- -5' |
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| 19098 | 3' | -53.3 | NC_004684.1 | + | 58632 | 0.77 | 0.270314 |
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Target: 5'- cGGCUGGU-GCCGCGCu--GGGCCUCu -3' miRNA: 3'- -CUGACCAuUGGCGCGuuuUCUGGGGc -5' |
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| 19098 | 3' | -53.3 | NC_004684.1 | + | 61180 | 0.67 | 0.785939 |
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Target: 5'- aGCUGGccgaGAUCGCGCcGGAGGCCaUCGa -3' miRNA: 3'- cUGACCa---UUGGCGCGuUUUCUGG-GGC- -5' |
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| 19098 | 3' | -53.3 | NC_004684.1 | + | 61693 | 0.69 | 0.713473 |
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Target: 5'- uGGCgucGGU-GCCGUggGCAGGccGGGCCCCGg -3' miRNA: 3'- -CUGa--CCAuUGGCG--CGUUU--UCUGGGGC- -5' |
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| 19098 | 3' | -53.3 | NC_004684.1 | + | 62660 | 0.69 | 0.724158 |
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Target: 5'- cGGCU-GUGGCCGUGCGcGAGGUCCUGg -3' miRNA: 3'- -CUGAcCAUUGGCGCGUuUUCUGGGGC- -5' |
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| 19098 | 3' | -53.3 | NC_004684.1 | + | 62993 | 0.66 | 0.841971 |
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Target: 5'- uGGCcGGUGGCCgggaacgcgauGCGCAGGAuGACCgCGc -3' miRNA: 3'- -CUGaCCAUUGG-----------CGCGUUUU-CUGGgGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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