Results 61 - 68 of 68 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 19098 | 3' | -53.3 | NC_004684.1 | + | 5049 | 0.7 | 0.615024 |
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Target: 5'- aGGCUGGcgcgcGCUGUGCuGGAGGCCuCCGg -3' miRNA: 3'- -CUGACCau---UGGCGCGuUUUCUGG-GGC- -5' |
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| 19098 | 3' | -53.3 | NC_004684.1 | + | 27760 | 0.7 | 0.615024 |
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Target: 5'- ---aGGUGACCGUGCcgcgcGACCCCc -3' miRNA: 3'- cugaCCAUUGGCGCGuuuu-CUGGGGc -5' |
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| 19098 | 3' | -53.3 | NC_004684.1 | + | 48505 | 0.7 | 0.626037 |
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Target: 5'- cGACgUGGaggAGCCGCGCu--GGugCCCu -3' miRNA: 3'- -CUG-ACCa--UUGGCGCGuuuUCugGGGc -5' |
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| 19098 | 3' | -53.3 | NC_004684.1 | + | 7066 | 0.69 | 0.69296 |
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Target: 5'- ---cGGUGAUCGCGCucgaacgcauugccGAGGCCCUGg -3' miRNA: 3'- cugaCCAUUGGCGCGuu------------UUCUGGGGC- -5' |
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| 19098 | 3' | -53.3 | NC_004684.1 | + | 19236 | 0.69 | 0.702708 |
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Target: 5'- aACUGGgaGCCGCGCAAc--GCuCCCa -3' miRNA: 3'- cUGACCauUGGCGCGUUuucUG-GGGc -5' |
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| 19098 | 3' | -53.3 | NC_004684.1 | + | 62660 | 0.69 | 0.724158 |
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Target: 5'- cGGCU-GUGGCCGUGCGcGAGGUCCUGg -3' miRNA: 3'- -CUGAcCAUUGGCGCGUuUUCUGGGGC- -5' |
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| 19098 | 3' | -53.3 | NC_004684.1 | + | 14087 | 0.68 | 0.755617 |
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Target: 5'- cGACcagGGUGACCGgGUGGuguGGcucaGCCCCGg -3' miRNA: 3'- -CUGa--CCAUUGGCgCGUUu--UC----UGGGGC- -5' |
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| 19098 | 3' | -53.3 | NC_004684.1 | + | 10018 | 1.09 | 0.002181 |
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Target: 5'- cGACUGGUAACCGCGCAAAAGACCCCGc -3' miRNA: 3'- -CUGACCAUUGGCGCGUUUUCUGGGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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