Results 61 - 68 of 68 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 19098 | 3' | -53.3 | NC_004684.1 | + | 19464 | 0.67 | 0.824075 |
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Target: 5'- cGGC-GGUGGcCCGCGCGAcaugcGCCCCu -3' miRNA: 3'- -CUGaCCAUU-GGCGCGUUuuc--UGGGGc -5' |
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| 19098 | 3' | -53.3 | NC_004684.1 | + | 20016 | 0.67 | 0.814819 |
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Target: 5'- cACcGGaGGCCGUGguGccugAAGACCCCGc -3' miRNA: 3'- cUGaCCaUUGGCGCguU----UUCUGGGGC- -5' |
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| 19098 | 3' | -53.3 | NC_004684.1 | + | 39796 | 0.67 | 0.813882 |
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Target: 5'- cGCUGGUAGCUGCGCcGguccaugucggacAGGugCuuGg -3' miRNA: 3'- cUGACCAUUGGCGCGuU-------------UUCugGggC- -5' |
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| 19098 | 3' | -53.3 | NC_004684.1 | + | 58354 | 0.67 | 0.812944 |
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Target: 5'- uGCUGGcguuGCCGCGCcgguugccGCCCCGc -3' miRNA: 3'- cUGACCau--UGGCGCGuuuuc---UGGGGC- -5' |
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| 19098 | 3' | -53.3 | NC_004684.1 | + | 33297 | 0.67 | 0.80537 |
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Target: 5'- --gUGGUGcUCGCGCGGGuGGCCUCGc -3' miRNA: 3'- cugACCAUuGGCGCGUUUuCUGGGGC- -5' |
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| 19098 | 3' | -53.3 | NC_004684.1 | + | 56851 | 0.67 | 0.785939 |
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Target: 5'- aGCUGGUGgagcACCacgaggucaGCGCAcccuGGCCCCGc -3' miRNA: 3'- cUGACCAU----UGG---------CGCGUuuu-CUGGGGC- -5' |
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| 19098 | 3' | -53.3 | NC_004684.1 | + | 55644 | 0.67 | 0.785939 |
|
Target: 5'- cGGCUGGcGugCGUGgAGGAGGgCCUGg -3' miRNA: 3'- -CUGACCaUugGCGCgUUUUCUgGGGC- -5' |
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| 19098 | 3' | -53.3 | NC_004684.1 | + | 58632 | 0.77 | 0.270314 |
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Target: 5'- cGGCUGGU-GCCGCGCu--GGGCCUCu -3' miRNA: 3'- -CUGACCAuUGGCGCGuuuUCUGGGGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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