Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 19098 | 5' | -54.8 | NC_004684.1 | + | 54602 | 0.67 | 0.73708 |
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Target: 5'- cGGCGGGGgugCcgUUGUCcucGU-UGGCCg -3' miRNA: 3'- -CCGCCCCa--GaaAACAGa--CAcACCGGa -5' |
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| 19098 | 5' | -54.8 | NC_004684.1 | + | 57367 | 0.67 | 0.705396 |
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Target: 5'- gGGCGGGGUCUUUUGcgCgGUuaccaGUcgcuauccaggcGGCCa -3' miRNA: 3'- -CCGCCCCAGAAAACa-GaCA-----CA------------CCGGa -5' |
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| 19098 | 5' | -54.8 | NC_004684.1 | + | 39908 | 0.68 | 0.683902 |
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Target: 5'- cGGCGGGuUC-UUUGUCguUGUGUcGGUCa -3' miRNA: 3'- -CCGCCCcAGaAAACAG--ACACA-CCGGa -5' |
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| 19098 | 5' | -54.8 | NC_004684.1 | + | 62814 | 0.68 | 0.650232 |
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Target: 5'- aGGCGGGGgagCUUUcaggccgaccgggUGUCggugaugGUGUagcgGGCCUc -3' miRNA: 3'- -CCGCCCCa--GAAA-------------ACAGa------CACA----CCGGA- -5' |
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| 19098 | 5' | -54.8 | NC_004684.1 | + | 16758 | 0.69 | 0.607683 |
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Target: 5'- cGGCGGuGUCgacugGuUCUGUGUcGGCCa -3' miRNA: 3'- -CCGCCcCAGaaaa-C-AGACACA-CCGGa -5' |
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| 19098 | 5' | -54.8 | NC_004684.1 | + | 10052 | 1.11 | 0.001046 |
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Target: 5'- cGGCGGGGUCUUUUGUCUGUGUGGCCUa -3' miRNA: 3'- -CCGCCCCAGAAAACAGACACACCGGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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