Results 81 - 88 of 88 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 19100 | 5' | -57.3 | NC_004684.1 | + | 42415 | 0.66 | 0.661957 |
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Target: 5'- cGCCGaCGCGGGCCaagGUaugggcaggacuggCGGUCAucGCGc -3' miRNA: 3'- -CGGCgGUGCCCGG---CA--------------GCUAGUuuCGU- -5' |
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| 19100 | 5' | -57.3 | NC_004684.1 | + | 45091 | 0.66 | 0.654495 |
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Target: 5'- cGCCGCCAgGaGGCCGacuugUCGuUgAAGGUg -3' miRNA: 3'- -CGGCGGUgC-CCGGC-----AGCuAgUUUCGu -5' |
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| 19100 | 5' | -57.3 | NC_004684.1 | + | 3602 | 0.66 | 0.675777 |
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Target: 5'- cGUCGCgCACuGGGCCGcCGAacacgaguggcCGAAGCGc -3' miRNA: 3'- -CGGCG-GUG-CCCGGCaGCUa----------GUUUCGU- -5' |
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| 19100 | 5' | -57.3 | NC_004684.1 | + | 12450 | 0.66 | 0.643821 |
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Target: 5'- --gGCUGCGGGCCGgggagcaaUCGAcCGAGGUAc -3' miRNA: 3'- cggCGGUGCCCGGC--------AGCUaGUUUCGU- -5' |
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| 19100 | 5' | -57.3 | NC_004684.1 | + | 47606 | 0.66 | 0.675777 |
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Target: 5'- gGCCGCaCACc-GCCGUCGGUaCucGGCGu -3' miRNA: 3'- -CGGCG-GUGccCGGCAGCUA-GuuUCGU- -5' |
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| 19100 | 5' | -57.3 | NC_004684.1 | + | 36761 | 0.66 | 0.654495 |
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Target: 5'- cGCCGCCA-GGGCCGcgccCGGUgcCGAcGCu -3' miRNA: 3'- -CGGCGGUgCCCGGCa---GCUA--GUUuCGu -5' |
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| 19100 | 5' | -57.3 | NC_004684.1 | + | 55560 | 0.66 | 0.643821 |
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Target: 5'- gGCCGCCGuggccCGguGGCCGggGGUCcAGGCAc -3' miRNA: 3'- -CGGCGGU-----GC--CCGGCagCUAGuUUCGU- -5' |
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| 19100 | 5' | -57.3 | NC_004684.1 | + | 4013 | 0.66 | 0.686364 |
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Target: 5'- cGCCGCCAuCGgcuggcgcgucGGCCGcUCGGUgCGcuGGGCGg -3' miRNA: 3'- -CGGCGGU-GC-----------CCGGC-AGCUA-GU--UUCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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