Results 81 - 88 of 88 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 19100 | 5' | -57.3 | NC_004684.1 | + | 40050 | 0.72 | 0.318429 |
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Target: 5'- uGCCGgugcCCACGGcgauaccGCCGUCG-UCAAAGCu -3' miRNA: 3'- -CGGC----GGUGCC-------CGGCAGCuAGUUUCGu -5' |
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| 19100 | 5' | -57.3 | NC_004684.1 | + | 55305 | 0.73 | 0.304253 |
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Target: 5'- gGCCGCCgcGCGGGCCagcaccucGUCGcgCucGGCGg -3' miRNA: 3'- -CGGCGG--UGCCCGG--------CAGCuaGuuUCGU- -5' |
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| 19100 | 5' | -57.3 | NC_004684.1 | + | 65581 | 0.73 | 0.296989 |
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Target: 5'- aGCCGCCAU-GGCC-UCGGUCAuGAGCc -3' miRNA: 3'- -CGGCGGUGcCCGGcAGCUAGU-UUCGu -5' |
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| 19100 | 5' | -57.3 | NC_004684.1 | + | 245 | 0.73 | 0.296989 |
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Target: 5'- cGCCGCCACGaucaucgugcaGGCCGaCGG-CGAGGCc -3' miRNA: 3'- -CGGCGGUGC-----------CCGGCaGCUaGUUUCGu -5' |
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| 19100 | 5' | -57.3 | NC_004684.1 | + | 26808 | 0.73 | 0.29627 |
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Target: 5'- -aCGCCACGGGCCgGUUGAUgAAcgacaccAGCGc -3' miRNA: 3'- cgGCGGUGCCCGG-CAGCUAgUU-------UCGU- -5' |
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| 19100 | 5' | -57.3 | NC_004684.1 | + | 67003 | 0.74 | 0.262687 |
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Target: 5'- cCCGUaCGCGGgGCCGUCGGUCucGGCc -3' miRNA: 3'- cGGCG-GUGCC-CGGCAGCUAGuuUCGu -5' |
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| 19100 | 5' | -57.3 | NC_004684.1 | + | 59857 | 0.82 | 0.072786 |
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Target: 5'- cGCCGCCACGgcGGCCagGUCGGUCcGGGCAg -3' miRNA: 3'- -CGGCGGUGC--CCGG--CAGCUAGuUUCGU- -5' |
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| 19100 | 5' | -57.3 | NC_004684.1 | + | 8779 | 1.1 | 0.000724 |
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Target: 5'- aGCCGCCACGGGCCGUCGAUCAAAGCAc -3' miRNA: 3'- -CGGCGGUGCCCGGCAGCUAGUUUCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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