Results 81 - 88 of 88 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 19100 | 5' | -57.3 | NC_004684.1 | + | 65067 | 0.71 | 0.401053 |
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Target: 5'- cGCCGCCaguucguccugcGCGGugaucauGCCGUCGAgCAGGGUg -3' miRNA: 3'- -CGGCGG------------UGCC-------CGGCAGCUaGUUUCGu -5' |
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| 19100 | 5' | -57.3 | NC_004684.1 | + | 65581 | 0.73 | 0.296989 |
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Target: 5'- aGCCGCCAU-GGCC-UCGGUCAuGAGCc -3' miRNA: 3'- -CGGCGGUGcCCGGcAGCUAGU-UUCGu -5' |
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| 19100 | 5' | -57.3 | NC_004684.1 | + | 65767 | 0.71 | 0.375728 |
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Target: 5'- gGCCGCCGCGcGCCGgugggcgCGcgCAcGGCGa -3' miRNA: 3'- -CGGCGGUGCcCGGCa------GCuaGUuUCGU- -5' |
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| 19100 | 5' | -57.3 | NC_004684.1 | + | 66173 | 0.66 | 0.675777 |
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Target: 5'- gGCCGCCGa-GGCCGaCGAagUCGAcGCc -3' miRNA: 3'- -CGGCGGUgcCCGGCaGCU--AGUUuCGu -5' |
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| 19100 | 5' | -57.3 | NC_004684.1 | + | 66650 | 0.71 | 0.393077 |
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Target: 5'- cGCCGCCA-GGG-CGUCGGugucgUCGGGGUAg -3' miRNA: 3'- -CGGCGGUgCCCgGCAGCU-----AGUUUCGU- -5' |
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| 19100 | 5' | -57.3 | NC_004684.1 | + | 66692 | 0.72 | 0.345827 |
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Target: 5'- gGCCGuCCACcggcggcugaccucgGGGCCGagGAUCAGGcGCAc -3' miRNA: 3'- -CGGC-GGUG---------------CCCGGCagCUAGUUU-CGU- -5' |
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| 19100 | 5' | -57.3 | NC_004684.1 | + | 67003 | 0.74 | 0.262687 |
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Target: 5'- cCCGUaCGCGGgGCCGUCGGUCucGGCc -3' miRNA: 3'- cGGCG-GUGCC-CGGCAGCUAGuuUCGu -5' |
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| 19100 | 5' | -57.3 | NC_004684.1 | + | 67061 | 0.66 | 0.643821 |
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Target: 5'- cCCGCCAacaccucguUGGGCCGccCGGUCuuGGUc -3' miRNA: 3'- cGGCGGU---------GCCCGGCa-GCUAGuuUCGu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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