Results 81 - 88 of 88 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 19100 | 5' | -57.3 | NC_004684.1 | + | 3710 | 0.7 | 0.438654 |
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Target: 5'- cGCCGCCGCGcuGGCCGgu-AUCAAcGCu -3' miRNA: 3'- -CGGCGGUGC--CCGGCagcUAGUUuCGu -5' |
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| 19100 | 5' | -57.3 | NC_004684.1 | + | 3652 | 0.67 | 0.633136 |
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Target: 5'- gGCCGCC-CGGuGCCG--GGUCAucGUAc -3' miRNA: 3'- -CGGCGGuGCC-CGGCagCUAGUuuCGU- -5' |
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| 19100 | 5' | -57.3 | NC_004684.1 | + | 3602 | 0.66 | 0.675777 |
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Target: 5'- cGUCGCgCACuGGGCCGcCGAacacgaguggcCGAAGCGc -3' miRNA: 3'- -CGGCG-GUG-CCCGGCaGCUa----------GUUUCGU- -5' |
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| 19100 | 5' | -57.3 | NC_004684.1 | + | 3519 | 0.68 | 0.548337 |
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Target: 5'- aUCGCCGacgggUGGGCCGaccuggCGGUgCAAGGCAa -3' miRNA: 3'- cGGCGGU-----GCCCGGCa-----GCUA-GUUUCGU- -5' |
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| 19100 | 5' | -57.3 | NC_004684.1 | + | 862 | 0.67 | 0.600046 |
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Target: 5'- aCCGCCGugcccgcCGGGCCG-CGcUgAAGGCGg -3' miRNA: 3'- cGGCGGU-------GCCCGGCaGCuAgUUUCGU- -5' |
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| 19100 | 5' | -57.3 | NC_004684.1 | + | 732 | 0.67 | 0.601111 |
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Target: 5'- aCCGCCGgugacCGGGCCGcCGc-CGAGGCc -3' miRNA: 3'- cGGCGGU-----GCCCGGCaGCuaGUUUCGu -5' |
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| 19100 | 5' | -57.3 | NC_004684.1 | + | 518 | 0.66 | 0.666215 |
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Target: 5'- gGUCGCCAcCGGcGCUGacgugcuggccagcgCGGUCGAGGCc -3' miRNA: 3'- -CGGCGGU-GCC-CGGCa--------------GCUAGUUUCGu -5' |
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| 19100 | 5' | -57.3 | NC_004684.1 | + | 245 | 0.73 | 0.296989 |
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Target: 5'- cGCCGCCACGaucaucgugcaGGCCGaCGG-CGAGGCc -3' miRNA: 3'- -CGGCGGUGC-----------CCGGCaGCUaGUUUCGu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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