Results 81 - 88 of 88 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 19100 | 5' | -57.3 | NC_004684.1 | + | 34126 | 0.68 | 0.527613 |
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Target: 5'- uGCuCGUCGCGGuGCUGUUGAgCGAcAGCAc -3' miRNA: 3'- -CG-GCGGUGCC-CGGCAGCUaGUU-UCGU- -5' |
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| 19100 | 5' | -57.3 | NC_004684.1 | + | 58305 | 0.68 | 0.51736 |
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Target: 5'- -gCGUCACGGGCCucGUCGGUCuu-GUc -3' miRNA: 3'- cgGCGGUGCCCGG--CAGCUAGuuuCGu -5' |
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| 19100 | 5' | -57.3 | NC_004684.1 | + | 11225 | 0.69 | 0.507187 |
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Target: 5'- cGCCGCCGuCGucGCCGUCGucggcCAGGGCc -3' miRNA: 3'- -CGGCGGU-GCc-CGGCAGCua---GUUUCGu -5' |
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| 19100 | 5' | -57.3 | NC_004684.1 | + | 20802 | 0.69 | 0.497101 |
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Target: 5'- cGCCGCCGuCGccGCCGUCGAgcgCGAcugggaauccGGCAc -3' miRNA: 3'- -CGGCGGU-GCc-CGGCAGCUa--GUU----------UCGU- -5' |
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| 19100 | 5' | -57.3 | NC_004684.1 | + | 18075 | 0.69 | 0.497101 |
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Target: 5'- gGCCGCCgACGGGUCGauccuggaCGG-CAAGGCc -3' miRNA: 3'- -CGGCGG-UGCCCGGCa-------GCUaGUUUCGu -5' |
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| 19100 | 5' | -57.3 | NC_004684.1 | + | 28558 | 0.69 | 0.467407 |
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Target: 5'- cGCCaggGCCACcaGGCCGUCGggCAccgccAGGCGg -3' miRNA: 3'- -CGG---CGGUGc-CCGGCAGCuaGU-----UUCGU- -5' |
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| 19100 | 5' | -57.3 | NC_004684.1 | + | 53473 | 0.69 | 0.466432 |
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Target: 5'- aGCCGCCAgccagcugucgauCuGGCCGUCGGUgAucGCc -3' miRNA: 3'- -CGGCGGU-------------GcCCGGCAGCUAgUuuCGu -5' |
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| 19100 | 5' | -57.3 | NC_004684.1 | + | 8779 | 1.1 | 0.000724 |
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Target: 5'- aGCCGCCACGGGCCGUCGAUCAAAGCAc -3' miRNA: 3'- -CGGCGGUGCCCGGCAGCUAGUUUCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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