Results 81 - 88 of 88 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 19100 | 5' | -57.3 | NC_004684.1 | + | 28086 | 0.68 | 0.56931 |
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Target: 5'- gGCCGCCGCgcgacccgcaccGGGUgGUgCGGUuCGAGGCc -3' miRNA: 3'- -CGGCGGUG------------CCCGgCA-GCUA-GUUUCGu -5' |
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| 19100 | 5' | -57.3 | NC_004684.1 | + | 24775 | 0.67 | 0.579872 |
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Target: 5'- aCCGgCAcCGGGCCGcuggUCGAUacggcCAAGGCGg -3' miRNA: 3'- cGGCgGU-GCCCGGC----AGCUA-----GUUUCGU- -5' |
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| 19100 | 5' | -57.3 | NC_004684.1 | + | 10160 | 0.67 | 0.590475 |
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Target: 5'- aGCCGCCGCuGGCCaccgCGcUCGAGGa- -3' miRNA: 3'- -CGGCGGUGcCCGGca--GCuAGUUUCgu -5' |
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| 19100 | 5' | -57.3 | NC_004684.1 | + | 62306 | 0.67 | 0.590475 |
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Target: 5'- cGCCG-CGCGGGCCuccgCGAUCucaucgccgGAGGCc -3' miRNA: 3'- -CGGCgGUGCCCGGca--GCUAG---------UUUCGu -5' |
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| 19100 | 5' | -57.3 | NC_004684.1 | + | 10499 | 0.67 | 0.601111 |
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Target: 5'- gGCCGaCgCACuGGGCCGccgaCGGUCAgcGCGc -3' miRNA: 3'- -CGGC-G-GUG-CCCGGCa---GCUAGUuuCGU- -5' |
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| 19100 | 5' | -57.3 | NC_004684.1 | + | 45311 | 0.67 | 0.601111 |
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Target: 5'- aGCCGUCGCu-GCCGUUGAUgAAcGGCAc -3' miRNA: 3'- -CGGCGGUGccCGGCAGCUAgUU-UCGU- -5' |
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| 19100 | 5' | -57.3 | NC_004684.1 | + | 48346 | 0.67 | 0.611772 |
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Target: 5'- cGCUGgauCCACGGGCgGUC--UCGGAGUg -3' miRNA: 3'- -CGGC---GGUGCCCGgCAGcuAGUUUCGu -5' |
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| 19100 | 5' | -57.3 | NC_004684.1 | + | 47606 | 0.66 | 0.675777 |
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Target: 5'- gGCCGCaCACc-GCCGUCGGUaCucGGCGu -3' miRNA: 3'- -CGGCG-GUGccCGGCAGCUA-GuuUCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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