Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 19104 | 5' | -61.1 | NC_004684.1 | + | 22349 | 0.66 | 0.44766 |
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Target: 5'- aGGCGgugGCGGcGgcgGCGgcGGUCGCGCUg -3' miRNA: 3'- -UCGCa--CGCC-CagaCGC--CCAGUGCGAg -5' |
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| 19104 | 5' | -61.1 | NC_004684.1 | + | 65954 | 0.66 | 0.44766 |
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Target: 5'- gAGUGUGCaccuucguGGaCaGCGGGUCGCGCa- -3' miRNA: 3'- -UCGCACGc-------CCaGaCGCCCAGUGCGag -5' |
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| 19104 | 5' | -61.1 | NC_004684.1 | + | 56617 | 0.67 | 0.393591 |
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Target: 5'- cGcCGUcGCGGGUgUcGCGGG-CACGCa- -3' miRNA: 3'- uC-GCA-CGCCCAgA-CGCCCaGUGCGag -5' |
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| 19104 | 5' | -61.1 | NC_004684.1 | + | 67175 | 0.67 | 0.384999 |
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Target: 5'- cGGCaugGUGCcGGaCgUGCGGGUCACGgUCu -3' miRNA: 3'- -UCG---CACGcCCaG-ACGCCCAGUGCgAG- -5' |
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| 19104 | 5' | -61.1 | NC_004684.1 | + | 38985 | 0.69 | 0.32086 |
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Target: 5'- cGCGcGCGGGUCcaacacCGGcGUCACGCa- -3' miRNA: 3'- uCGCaCGCCCAGac----GCC-CAGUGCGag -5' |
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| 19104 | 5' | -61.1 | NC_004684.1 | + | 13766 | 0.69 | 0.306128 |
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Target: 5'- cAGCGcaUGCGGGUgUcCGGGcCGCGCg- -3' miRNA: 3'- -UCGC--ACGCCCAgAcGCCCaGUGCGag -5' |
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| 19104 | 5' | -61.1 | NC_004684.1 | + | 951 | 0.7 | 0.26121 |
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Target: 5'- cGCuucGCGGGcCUGCGGGUCauuggcgacuccgggGCGUUCu -3' miRNA: 3'- uCGca-CGCCCaGACGCCCAG---------------UGCGAG- -5' |
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| 19104 | 5' | -61.1 | NC_004684.1 | + | 64983 | 0.7 | 0.252401 |
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Target: 5'- cGCGacGCGGGUCUGCGGcUCGCcaGCg- -3' miRNA: 3'- uCGCa-CGCCCAGACGCCcAGUG--CGag -5' |
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| 19104 | 5' | -61.1 | NC_004684.1 | + | 8156 | 1.09 | 0.000318 |
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Target: 5'- gAGCGUGCGGGUCUGCGGGUCACGCUCg -3' miRNA: 3'- -UCGCACGCCCAGACGCCCAGUGCGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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