Results 81 - 88 of 88 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 19113 | 3' | -55.2 | NC_004684.1 | + | 43635 | 0.66 | 0.776321 |
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Target: 5'- gCCGaGCGCGgUGAGUUCGucGGaCCAa- -3' miRNA: 3'- -GGUaCGCGUgGCUCAAGCu-CC-GGUac -5' |
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| 19113 | 3' | -55.2 | NC_004684.1 | + | 59451 | 0.66 | 0.786109 |
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Target: 5'- aCCgGUGUGCGCCGG--UCGGcGGCgAUGu -3' miRNA: 3'- -GG-UACGCGUGGCUcaAGCU-CCGgUAC- -5' |
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| 19113 | 3' | -55.2 | NC_004684.1 | + | 60065 | 0.66 | 0.786109 |
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Target: 5'- --uUGCaCACCGAGcacgCGucGGCCAUGg -3' miRNA: 3'- gguACGcGUGGCUCaa--GCu-CCGGUAC- -5' |
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| 19113 | 3' | -55.2 | NC_004684.1 | + | 5815 | 0.66 | 0.789982 |
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Target: 5'- aCGUGcCGCACCgugGAGUacaagcuggcgguguUCGAGGaCCAg- -3' miRNA: 3'- gGUAC-GCGUGG---CUCA---------------AGCUCC-GGUac -5' |
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| 19113 | 3' | -55.2 | NC_004684.1 | + | 45544 | 0.66 | 0.795743 |
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Target: 5'- aCcgGCGCACCGGGggacAGGUCGg- -3' miRNA: 3'- gGuaCGCGUGGCUCaagcUCCGGUac -5' |
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| 19113 | 3' | -55.2 | NC_004684.1 | + | 66580 | 0.66 | 0.795743 |
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Target: 5'- uCCAUGCGgGCCGccacggcgaGGUgcaggUGAgGGCCAUc -3' miRNA: 3'- -GGUACGCgUGGC---------UCAa----GCU-CCGGUAc -5' |
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| 19113 | 3' | -55.2 | NC_004684.1 | + | 742 | 0.66 | 0.795743 |
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Target: 5'- aCCggGcCGcCGCCGAGgccaUCGuGGCCAc- -3' miRNA: 3'- -GGuaC-GC-GUGGCUCa---AGCuCCGGUac -5' |
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| 19113 | 3' | -55.2 | NC_004684.1 | + | 35855 | 0.66 | 0.795743 |
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Target: 5'- uCCAguucGCGggcagggaACCGAgGUUCGcgcGGGCCGUGg -3' miRNA: 3'- -GGUa---CGCg-------UGGCU-CAAGC---UCCGGUAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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