Results 81 - 88 of 88 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 19113 | 3' | -55.2 | NC_004684.1 | + | 36479 | 0.75 | 0.305416 |
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Target: 5'- -aGUGCgGCACCGGGUUCGAcaucggcaaGGCCGg- -3' miRNA: 3'- ggUACG-CGUGGCUCAAGCU---------CCGGUac -5' |
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| 19113 | 3' | -55.2 | NC_004684.1 | + | 53730 | 0.76 | 0.263192 |
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Target: 5'- gCAUG-GUGCCGGGUUCGAcGGCCAg- -3' miRNA: 3'- gGUACgCGUGGCUCAAGCU-CCGGUac -5' |
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| 19113 | 3' | -55.2 | NC_004684.1 | + | 13525 | 0.76 | 0.259241 |
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Target: 5'- cCCGUGCGCcgaCGAGUUCGAggagcugcgccuggcGGCCAUc -3' miRNA: 3'- -GGUACGCGug-GCUCAAGCU---------------CCGGUAc -5' |
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| 19113 | 3' | -55.2 | NC_004684.1 | + | 63169 | 0.76 | 0.25021 |
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Target: 5'- aCCAgguUGCGCGCCGGGUcgacUCGAcgcGGCCGa- -3' miRNA: 3'- -GGU---ACGCGUGGCUCA----AGCU---CCGGUac -5' |
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| 19113 | 3' | -55.2 | NC_004684.1 | + | 29725 | 0.77 | 0.208355 |
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Target: 5'- uCCAccugGCGCACCGGG-UCGAugccugaccgggcGGCCAUGg -3' miRNA: 3'- -GGUa---CGCGUGGCUCaAGCU-------------CCGGUAC- -5' |
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| 19113 | 3' | -55.2 | NC_004684.1 | + | 58782 | 0.79 | 0.16455 |
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Target: 5'- gCcgGUGCACCGGgcuuGUUCGAGGCCAc- -3' miRNA: 3'- gGuaCGCGUGGCU----CAAGCUCCGGUac -5' |
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| 19113 | 3' | -55.2 | NC_004684.1 | + | 26052 | 0.8 | 0.147695 |
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Target: 5'- gCCA-GgGCGgCGAGUUCGAGGCCAa- -3' miRNA: 3'- -GGUaCgCGUgGCUCAAGCUCCGGUac -5' |
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| 19113 | 3' | -55.2 | NC_004684.1 | + | 3276 | 1.11 | 0.000924 |
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Target: 5'- aCCAUGCGCACCGAGUUCGAGGCCAUGg -3' miRNA: 3'- -GGUACGCGUGGCUCAAGCUCCGGUAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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