Results 81 - 88 of 88 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 19113 | 3' | -55.2 | NC_004684.1 | + | 63209 | 0.67 | 0.704367 |
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Target: 5'- aCCAccGCGCccGCCGGGcgCaaccGGGCCAUGa -3' miRNA: 3'- -GGUa-CGCG--UGGCUCaaGc---UCCGGUAC- -5' |
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| 19113 | 3' | -55.2 | NC_004684.1 | + | 63361 | 0.68 | 0.683031 |
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Target: 5'- cCCA-GCGCACCGAGcggcCGAcGcGCCAg- -3' miRNA: 3'- -GGUaCGCGUGGCUCaa--GCU-C-CGGUac -5' |
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| 19113 | 3' | -55.2 | NC_004684.1 | + | 64805 | 0.73 | 0.369494 |
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Target: 5'- gCCGUugGUGCugUcGAGUUCGuGGGCCAUGg -3' miRNA: 3'- -GGUA--CGCGugG-CUCAAGC-UCCGGUAC- -5' |
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| 19113 | 3' | -55.2 | NC_004684.1 | + | 64866 | 0.67 | 0.735832 |
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Target: 5'- -gGUGCGCagcguccgcaguGCCGGGcgucgUUGAGGCCGc- -3' miRNA: 3'- ggUACGCG------------UGGCUCa----AGCUCCGGUac -5' |
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| 19113 | 3' | -55.2 | NC_004684.1 | + | 65210 | 0.7 | 0.553797 |
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Target: 5'- gCCGUGCgGCACC----UCGcGGGCCAUGa -3' miRNA: 3'- -GGUACG-CGUGGcucaAGC-UCCGGUAC- -5' |
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| 19113 | 3' | -55.2 | NC_004684.1 | + | 66137 | 0.67 | 0.693728 |
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Target: 5'- gCCA-GCGCAUCuGGgcCGGGGCCGg- -3' miRNA: 3'- -GGUaCGCGUGGcUCaaGCUCCGGUac -5' |
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| 19113 | 3' | -55.2 | NC_004684.1 | + | 66285 | 0.67 | 0.746133 |
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Target: 5'- cUCGUGCcaguuGCGCCGGG-UCGcGGCC-UGg -3' miRNA: 3'- -GGUACG-----CGUGGCUCaAGCuCCGGuAC- -5' |
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| 19113 | 3' | -55.2 | NC_004684.1 | + | 66580 | 0.66 | 0.795743 |
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Target: 5'- uCCAUGCGgGCCGccacggcgaGGUgcaggUGAgGGCCAUc -3' miRNA: 3'- -GGUACGCgUGGC---------UCAa----GCU-CCGGUAc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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