Results 41 - 48 of 48 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 19113 | 5' | -57.8 | NC_004684.1 | + | 19102 | 0.7 | 0.380764 |
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Target: 5'- uGCGCGAaGGCcGGUGGGacgUGCGCAu- -3' miRNA: 3'- uCGCGCUgCUGcUCACCC---ACGCGUuc -5' |
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| 19113 | 5' | -57.8 | NC_004684.1 | + | 4775 | 0.71 | 0.355544 |
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Target: 5'- aAGCGCcACGGCuccgGGGUGCGCGAc -3' miRNA: 3'- -UCGCGcUGCUGcucaCCCACGCGUUc -5' |
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| 19113 | 5' | -57.8 | NC_004684.1 | + | 46298 | 0.71 | 0.347403 |
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Target: 5'- gAGCGCGuuGCGGCGcuUGGcGUGCGCcAGc -3' miRNA: 3'- -UCGCGC--UGCUGCucACC-CACGCGuUC- -5' |
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| 19113 | 5' | -57.8 | NC_004684.1 | + | 51580 | 0.71 | 0.331524 |
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Target: 5'- uGCGCGGCGGCGAccGGGUGgucCAAGg -3' miRNA: 3'- uCGCGCUGCUGCUcaCCCACgc-GUUC- -5' |
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| 19113 | 5' | -57.8 | NC_004684.1 | + | 42243 | 0.72 | 0.316185 |
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Target: 5'- cGGCGCGAaGACGuGGUGGGa-CGCGAGc -3' miRNA: 3'- -UCGCGCUgCUGC-UCACCCacGCGUUC- -5' |
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| 19113 | 5' | -57.8 | NC_004684.1 | + | 5030 | 0.76 | 0.177262 |
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Target: 5'- -cCGCGACGACGA---GGUGCGCAGGc -3' miRNA: 3'- ucGCGCUGCUGCUcacCCACGCGUUC- -5' |
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| 19113 | 5' | -57.8 | NC_004684.1 | + | 53762 | 0.76 | 0.177262 |
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Target: 5'- uGCGCGGCGGCGAac--GUGCGCAGGa -3' miRNA: 3'- uCGCGCUGCUGCUcaccCACGCGUUC- -5' |
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| 19113 | 5' | -57.8 | NC_004684.1 | + | 3312 | 1.09 | 0.000725 |
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Target: 5'- gAGCGCGACGACGAGUGGGUGCGCAAGg -3' miRNA: 3'- -UCGCGCUGCUGCUCACCCACGCGUUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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