Results 41 - 48 of 48 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 19113 | 5' | -57.8 | NC_004684.1 | + | 56993 | 0.67 | 0.543662 |
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Target: 5'- gAGCGCGucaccgccgGgGACGAGcgcgccaucgGGGUGCGCuGGu -3' miRNA: 3'- -UCGCGC---------UgCUGCUCa---------CCCACGCGuUC- -5' |
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| 19113 | 5' | -57.8 | NC_004684.1 | + | 58658 | 0.68 | 0.533317 |
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Target: 5'- gGGCGCGGCGGCGAacaucGGGaucaGCGCc-- -3' miRNA: 3'- -UCGCGCUGCUGCUca---CCCa---CGCGuuc -5' |
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| 19113 | 5' | -57.8 | NC_004684.1 | + | 61335 | 0.68 | 0.492707 |
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Target: 5'- cAGCGCGGCGugGuuggucAGcUGGGc-CGCGAGg -3' miRNA: 3'- -UCGCGCUGCugC------UC-ACCCacGCGUUC- -5' |
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| 19113 | 5' | -57.8 | NC_004684.1 | + | 62645 | 0.67 | 0.596221 |
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Target: 5'- aGGuCGCGGCacuuGCGGcuGUGGccGUGCGCGAGg -3' miRNA: 3'- -UC-GCGCUGc---UGCU--CACC--CACGCGUUC- -5' |
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| 19113 | 5' | -57.8 | NC_004684.1 | + | 62954 | 0.7 | 0.380764 |
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Target: 5'- cGCGCGcCGACGAugGUGgcgugcucgguGGUGCGCAc- -3' miRNA: 3'- uCGCGCuGCUGCU--CAC-----------CCACGCGUuc -5' |
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| 19113 | 5' | -57.8 | NC_004684.1 | + | 63839 | 0.67 | 0.543662 |
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Target: 5'- uGGCGCGGCGcACGcugucGGUGccacGUGCGCGGu -3' miRNA: 3'- -UCGCGCUGC-UGC-----UCACc---CACGCGUUc -5' |
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| 19113 | 5' | -57.8 | NC_004684.1 | + | 64541 | 0.7 | 0.407151 |
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Target: 5'- cGGCuCGGCGGCcuuggugaccGGGUuGGUGCGCAGGa -3' miRNA: 3'- -UCGcGCUGCUG----------CUCAcCCACGCGUUC- -5' |
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| 19113 | 5' | -57.8 | NC_004684.1 | + | 64850 | 0.66 | 0.649481 |
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Target: 5'- aGGCGgGugGuuguggUGGG-GGGUGCGCGu- -3' miRNA: 3'- -UCGCgCugCu-----GCUCaCCCACGCGUuc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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