Results 41 - 48 of 48 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 19113 | 5' | -57.8 | NC_004684.1 | + | 5317 | 0.69 | 0.482773 |
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Target: 5'- cGCGCuGGCGACcgugcagcAGUGGGUgGCGguGGg -3' miRNA: 3'- uCGCG-CUGCUGc-------UCACCCA-CGCguUC- -5' |
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| 19113 | 5' | -57.8 | NC_004684.1 | + | 5030 | 0.76 | 0.177262 |
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Target: 5'- -cCGCGACGACGA---GGUGCGCAGGc -3' miRNA: 3'- ucGCGCUGCUGCUcacCCACGCGUUC- -5' |
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| 19113 | 5' | -57.8 | NC_004684.1 | + | 4775 | 0.71 | 0.355544 |
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Target: 5'- aAGCGCcACGGCuccgGGGUGCGCGAc -3' miRNA: 3'- -UCGCGcUGCUGcucaCCCACGCGUUc -5' |
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| 19113 | 5' | -57.8 | NC_004684.1 | + | 3312 | 1.09 | 0.000725 |
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Target: 5'- gAGCGCGACGACGAGUGGGUGCGCAAGg -3' miRNA: 3'- -UCGCGCUGCUGCUCACCCACGCGUUC- -5' |
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| 19113 | 5' | -57.8 | NC_004684.1 | + | 3115 | 0.66 | 0.635624 |
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Target: 5'- uGCGCG-CGGucaucucccccguuCGGGUGGuGUGCGCc-- -3' miRNA: 3'- uCGCGCuGCU--------------GCUCACC-CACGCGuuc -5' |
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| 19113 | 5' | -57.8 | NC_004684.1 | + | 2264 | 0.67 | 0.561393 |
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Target: 5'- cAGCGCGGCGGCGGGcauugccgccagccUGGGccacGaCGCGGu -3' miRNA: 3'- -UCGCGCUGCUGCUC--------------ACCCa---C-GCGUUc -5' |
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| 19113 | 5' | -57.8 | NC_004684.1 | + | 1487 | 0.66 | 0.660125 |
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Target: 5'- cGGUGCGGCGGCccuGGUGGccaaccUGCGCGc- -3' miRNA: 3'- -UCGCGCUGCUGc--UCACCc-----ACGCGUuc -5' |
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| 19113 | 5' | -57.8 | NC_004684.1 | + | 618 | 0.66 | 0.664376 |
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Target: 5'- aAGgGCGGCGAguaccgcgcCGAGgucgccgGGGUgcuguaccgggugguGCGCAAGa -3' miRNA: 3'- -UCgCGCUGCU---------GCUCa------CCCA---------------CGCGUUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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