Results 41 - 48 of 48 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 19113 | 5' | -57.8 | NC_004684.1 | + | 12077 | 0.66 | 0.617498 |
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Target: 5'- gAGCGCGACGAgGuGcUGGcccGCGCGGc -3' miRNA: 3'- -UCGCGCUGCUgCuC-ACCca-CGCGUUc -5' |
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| 19113 | 5' | -57.8 | NC_004684.1 | + | 16789 | 0.66 | 0.606849 |
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Target: 5'- aGGUGCGGCGcguCGuguGGUGGGcGCGCc-- -3' miRNA: 3'- -UCGCGCUGCu--GC---UCACCCaCGCGuuc -5' |
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| 19113 | 5' | -57.8 | NC_004684.1 | + | 53791 | 0.67 | 0.564539 |
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Target: 5'- cGGCGCGGCGcUGGGaucGGGuUGCGCc-- -3' miRNA: 3'- -UCGCGCUGCuGCUCa--CCC-ACGCGuuc -5' |
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| 19113 | 5' | -57.8 | NC_004684.1 | + | 28249 | 0.67 | 0.543662 |
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Target: 5'- cAGCGCGGuggaccCGGCcAGccuGGUGCGCGAGg -3' miRNA: 3'- -UCGCGCU------GCUGcUCac-CCACGCGUUC- -5' |
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| 19113 | 5' | -57.8 | NC_004684.1 | + | 63839 | 0.67 | 0.543662 |
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Target: 5'- uGGCGCGGCGcACGcugucGGUGccacGUGCGCGGu -3' miRNA: 3'- -UCGCGCUGC-UGC-----UCACc---CACGCGUUc -5' |
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| 19113 | 5' | -57.8 | NC_004684.1 | + | 56993 | 0.67 | 0.543662 |
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Target: 5'- gAGCGCGucaccgccgGgGACGAGcgcgccaucgGGGUGCGCuGGu -3' miRNA: 3'- -UCGCGC---------UgCUGCUCa---------CCCACGCGuUC- -5' |
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| 19113 | 5' | -57.8 | NC_004684.1 | + | 26580 | 0.67 | 0.543662 |
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Target: 5'- uGGCGCG-CGGCG-GU--GUGCGCGGGu -3' miRNA: 3'- -UCGCGCuGCUGCuCAccCACGCGUUC- -5' |
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| 19113 | 5' | -57.8 | NC_004684.1 | + | 42243 | 0.72 | 0.316185 |
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Target: 5'- cGGCGCGAaGACGuGGUGGGa-CGCGAGc -3' miRNA: 3'- -UCGCGCUgCUGC-UCACCCacGCGUUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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