Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 19121 | 5' | -56 | NC_004684.1 | + | 18720 | 0.67 | 0.648964 |
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Target: 5'- gCU-CUACAu--GCCCggugaaggccucuCCGGCGCGGUGc -3' miRNA: 3'- -GAuGAUGUucuUGGG-------------GGCCGCGCCAU- -5' |
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| 19121 | 5' | -56 | NC_004684.1 | + | 17730 | 0.7 | 0.500457 |
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Target: 5'- -aACUGCGGucGCCCggaauCCGGCGCGGc- -3' miRNA: 3'- gaUGAUGUUcuUGGG-----GGCCGCGCCau -5' |
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| 19121 | 5' | -56 | NC_004684.1 | + | 13138 | 0.76 | 0.224291 |
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Target: 5'- -gGCUG-AGGAGCUguCCCGGCGCGGUGc -3' miRNA: 3'- gaUGAUgUUCUUGG--GGGCCGCGCCAU- -5' |
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| 19121 | 5' | -56 | NC_004684.1 | + | 11818 | 0.68 | 0.606568 |
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Target: 5'- uUGgUGCcuGGACCCCCGGCcacCGGg- -3' miRNA: 3'- gAUgAUGuuCUUGGGGGCCGc--GCCau -5' |
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| 19121 | 5' | -56 | NC_004684.1 | + | 11506 | 0.67 | 0.65005 |
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Target: 5'- --cCUGCugGGACUCCCGGCaccgcuCGGUGg -3' miRNA: 3'- gauGAUGuuCUUGGGGGCCGc-----GCCAU- -5' |
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| 19121 | 5' | -56 | NC_004684.1 | + | 6066 | 0.66 | 0.725117 |
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Target: 5'- cCUACgccgcCGAGGGCggcggCCCGGCgGCGGUGc -3' miRNA: 3'- -GAUGau---GUUCUUGg----GGGCCG-CGCCAU- -5' |
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| 19121 | 5' | -56 | NC_004684.1 | + | 4786 | 0.67 | 0.628303 |
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Target: 5'- ----aGCAAGcg-CCUCGGCGCGGUGg -3' miRNA: 3'- gaugaUGUUCuugGGGGCCGCGCCAU- -5' |
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| 19121 | 5' | -56 | NC_004684.1 | + | 522 | 0.68 | 0.563423 |
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Target: 5'- gCUGCUGCAAG-GCCuggaCCCGGCGaaaGGc- -3' miRNA: 3'- -GAUGAUGUUCuUGG----GGGCCGCg--CCau -5' |
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| 19121 | 5' | -56 | NC_004684.1 | + | 224 | 1.07 | 0.001395 |
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Target: 5'- cCUACUACAAGAACCCCCGGCGCGGUAa -3' miRNA: 3'- -GAUGAUGUUCUUGGGGGCCGCGCCAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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