Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1919 | 5' | -51.6 | NC_001347.2 | + | 128784 | 0.66 | 0.996832 |
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Target: 5'- cGACGGCGgccauggagCUGcacUCaCgcgGCCGUCACg -3' miRNA: 3'- -UUGCCGCaaaa-----GAC---AG-Ga--UGGCAGUG- -5' |
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| 1919 | 5' | -51.6 | NC_001347.2 | + | 165111 | 0.67 | 0.988804 |
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Target: 5'- uGCGGCGguggucgUUGUCgCUGCCGUgucCGCg -3' miRNA: 3'- uUGCCGCaaaa---GACAG-GAUGGCA---GUG- -5' |
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| 1919 | 5' | -51.6 | NC_001347.2 | + | 91290 | 0.67 | 0.988804 |
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Target: 5'- cGACGGCGgcUUCaGUaCC-GCCGUCGa -3' miRNA: 3'- -UUGCCGCaaAAGaCA-GGaUGGCAGUg -5' |
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| 1919 | 5' | -51.6 | NC_001347.2 | + | 64712 | 0.68 | 0.987317 |
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Target: 5'- -uCGGCGccaaCUGgcUCCuUACCGUCACa -3' miRNA: 3'- uuGCCGCaaaaGAC--AGG-AUGGCAGUG- -5' |
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| 1919 | 5' | -51.6 | NC_001347.2 | + | 190101 | 0.68 | 0.987317 |
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Target: 5'- aAACGGUGgcUUUCga-CCUAUCGUCGCc -3' miRNA: 3'- -UUGCCGCa-AAAGacaGGAUGGCAGUG- -5' |
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| 1919 | 5' | -51.6 | NC_001347.2 | + | 41361 | 0.68 | 0.979808 |
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Target: 5'- gGACGGCGUc-UCUGUCUgg-CGUCAg -3' miRNA: 3'- -UUGCCGCAaaAGACAGGaugGCAGUg -5' |
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| 1919 | 5' | -51.6 | NC_001347.2 | + | 127695 | 0.69 | 0.972305 |
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Target: 5'- uAACGGCGUUUUCcaaaaaacaacGUCCUccguugACaCGUCACc -3' miRNA: 3'- -UUGCCGCAAAAGa----------CAGGA------UG-GCAGUG- -5' |
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| 1919 | 5' | -51.6 | NC_001347.2 | + | 88266 | 0.69 | 0.966297 |
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Target: 5'- cGCGGCGUacgCUgGUCaCgucGCCGUCGCg -3' miRNA: 3'- uUGCCGCAaaaGA-CAG-Ga--UGGCAGUG- -5' |
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| 1919 | 5' | -51.6 | NC_001347.2 | + | 193120 | 0.7 | 0.959425 |
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Target: 5'- cACGGCGUcUUCc-UCCUcgucgucgccGCCGUCGCg -3' miRNA: 3'- uUGCCGCAaAAGacAGGA----------UGGCAGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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