Results 41 - 47 of 47 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
19209 | 3' | -59.5 | NC_004684.1 | + | 1400 | 0.66 | 0.525711 |
Target: 5'- gGCGGCUaccggUA-CGGGCgGCugAGCc -3' miRNA: 3'- aUGCCGAga---AUcGCCCGgCGugUCGc -5' |
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19209 | 3' | -59.5 | NC_004684.1 | + | 46305 | 0.66 | 0.525711 |
Target: 5'- uUGCGGCgCUUGGCGuGC-GC-CAGCGu -3' miRNA: 3'- -AUGCCGaGAAUCGCcCGgCGuGUCGC- -5' |
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19209 | 3' | -59.5 | NC_004684.1 | + | 9466 | 0.66 | 0.536033 |
Target: 5'- --gGGCgCUggagcUGGCGGGCaaGCGCAGCc -3' miRNA: 3'- augCCGaGA-----AUCGCCCGg-CGUGUCGc -5' |
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19209 | 3' | -59.5 | NC_004684.1 | + | 22621 | 0.66 | 0.536033 |
Target: 5'- uUACGGCgCUgugGGa-GGCCGCcgcCAGCGg -3' miRNA: 3'- -AUGCCGaGAa--UCgcCCGGCGu--GUCGC- -5' |
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19209 | 3' | -59.5 | NC_004684.1 | + | 47175 | 0.66 | 0.536033 |
Target: 5'- aGCGGC----GGCcaGGGCCGCcgcugcGCGGCGa -3' miRNA: 3'- aUGCCGagaaUCG--CCCGGCG------UGUCGC- -5' |
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19209 | 3' | -59.5 | NC_004684.1 | + | 52917 | 0.66 | 0.536033 |
Target: 5'- cACGGC----GGCGuGGCCGCAUuccaGGCa -3' miRNA: 3'- aUGCCGagaaUCGC-CCGGCGUG----UCGc -5' |
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19209 | 3' | -59.5 | NC_004684.1 | + | 58620 | 0.66 | 0.536033 |
Target: 5'- gACGGCcCgUGGCGGcugguGCCGCGCugGGCc -3' miRNA: 3'- aUGCCGaGaAUCGCC-----CGGCGUG--UCGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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