Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 19209 | 5' | -57.5 | NC_004684.1 | + | 27621 | 0.66 | 0.63693 |
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Target: 5'- aUCGaGaACGGCCaggacgccaUGCCACCCGGUc- -3' miRNA: 3'- gAGCaCaUGCUGG---------GCGGUGGGCCAau -5' |
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| 19209 | 5' | -57.5 | NC_004684.1 | + | 42171 | 0.68 | 0.562626 |
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Target: 5'- uUCGcGUccaGCGugCCggugaaGCCACCCGGUg- -3' miRNA: 3'- gAGCaCA---UGCugGG------CGGUGGGCCAau -5' |
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| 19209 | 5' | -57.5 | NC_004684.1 | + | 969 | 0.69 | 0.480916 |
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Target: 5'- gUCGUaccuGUACggcgggacgGACCCGCCGCCCGa--- -3' miRNA: 3'- gAGCA----CAUG---------CUGGGCGGUGGGCcaau -5' |
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| 19209 | 5' | -57.5 | NC_004684.1 | + | 28411 | 0.7 | 0.422674 |
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Target: 5'- --gGUGUugGACCCGCgcgcgaaCACCuCGGUg- -3' miRNA: 3'- gagCACAugCUGGGCG-------GUGG-GCCAau -5' |
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| 19209 | 5' | -57.5 | NC_004684.1 | + | 28331 | 0.71 | 0.370564 |
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Target: 5'- aUCGUGUugACG-CUgGCCACCCGGc-- -3' miRNA: 3'- gAGCACA--UGCuGGgCGGUGGGCCaau -5' |
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| 19209 | 5' | -57.5 | NC_004684.1 | + | 56576 | 0.73 | 0.299917 |
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Target: 5'- aCUCG-GUGCGGCCCaGCCGCCggaugccgcuaCGGUg- -3' miRNA: 3'- -GAGCaCAUGCUGGG-CGGUGG-----------GCCAau -5' |
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| 19209 | 5' | -57.5 | NC_004684.1 | + | 61490 | 0.77 | 0.154403 |
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Target: 5'- gUCGUGcGCGAUcagcgccgCCGCCACCCGGUc- -3' miRNA: 3'- gAGCACaUGCUG--------GGCGGUGGGCCAau -5' |
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| 19209 | 5' | -57.5 | NC_004684.1 | + | 66910 | 0.66 | 0.668882 |
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Target: 5'- gUCG-GUGCugccGACCuCGCUgGCCCGGUUGu -3' miRNA: 3'- gAGCaCAUG----CUGG-GCGG-UGGGCCAAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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