Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 19209 | 5' | -57.5 | NC_004684.1 | + | 28411 | 0.7 | 0.422674 |
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Target: 5'- --gGUGUugGACCCGCgcgcgaaCACCuCGGUg- -3' miRNA: 3'- gagCACAugCUGGGCG-------GUGG-GCCAau -5' |
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| 19209 | 5' | -57.5 | NC_004684.1 | + | 28331 | 0.71 | 0.370564 |
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Target: 5'- aUCGUGUugACG-CUgGCCACCCGGc-- -3' miRNA: 3'- gAGCACA--UGCuGGgCGGUGGGCCaau -5' |
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| 19209 | 5' | -57.5 | NC_004684.1 | + | 28579 | 0.71 | 0.353923 |
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Target: 5'- --gGUGUGCGGCggCUGCCugCCGGUc- -3' miRNA: 3'- gagCACAUGCUG--GGCGGugGGCCAau -5' |
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| 19209 | 5' | -57.5 | NC_004684.1 | + | 56576 | 0.73 | 0.299917 |
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Target: 5'- aCUCG-GUGCGGCCCaGCCGCCggaugccgcuaCGGUg- -3' miRNA: 3'- -GAGCaCAUGCUGGG-CGGUGG-----------GCCAau -5' |
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| 19209 | 5' | -57.5 | NC_004684.1 | + | 22328 | 0.73 | 0.26539 |
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Target: 5'- -cCGUG----GCCCGCCACCCGGUa- -3' miRNA: 3'- gaGCACaugcUGGGCGGUGGGCCAau -5' |
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| 19209 | 5' | -57.5 | NC_004684.1 | + | 61490 | 0.77 | 0.154403 |
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Target: 5'- gUCGUGcGCGAUcagcgccgCCGCCACCCGGUc- -3' miRNA: 3'- gAGCACaUGCUG--------GGCGGUGGGCCAau -5' |
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| 19209 | 5' | -57.5 | NC_004684.1 | + | 57621 | 0.77 | 0.150339 |
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Target: 5'- -gCG-GUACGGCCaGCCACCCGGUg- -3' miRNA: 3'- gaGCaCAUGCUGGgCGGUGGGCCAau -5' |
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| 19209 | 5' | -57.5 | NC_004684.1 | + | 37493 | 1.06 | 0.001282 |
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Target: 5'- cCUCGUGUACGACCCGCCACCCGGUUAc -3' miRNA: 3'- -GAGCACAUGCUGGGCGGUGGGCCAAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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