Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 19216 | 5' | -52.3 | NC_004684.1 | + | 26190 | 0.73 | 0.541437 |
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Target: 5'- -cGCUGGCguCUGGU-CGCCUUUGAu-- -3' miRNA: 3'- gaCGACCG--GACCAuGUGGAAACUucc -5' |
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| 19216 | 5' | -52.3 | NC_004684.1 | + | 15272 | 0.73 | 0.541437 |
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Target: 5'- aCUGgaGGCCUgcgcGGUGCACCUgcu-GGGc -3' miRNA: 3'- -GACgaCCGGA----CCAUGUGGAaacuUCC- -5' |
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| 19216 | 5' | -52.3 | NC_004684.1 | + | 65852 | 0.73 | 0.519939 |
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Target: 5'- uUGUgggGGCCgcUGGUGcCACCUcgGAGGGg -3' miRNA: 3'- gACGa--CCGG--ACCAU-GUGGAaaCUUCC- -5' |
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| 19216 | 5' | -52.3 | NC_004684.1 | + | 14562 | 0.73 | 0.505092 |
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Target: 5'- -cGCUGGacaCCUGGUGCGCCggggccaccgaGGAGGa -3' miRNA: 3'- gaCGACC---GGACCAUGUGGaaa--------CUUCC- -5' |
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| 19216 | 5' | -52.3 | NC_004684.1 | + | 61945 | 0.73 | 0.488351 |
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Target: 5'- -cGCcgGGCCUGGUGCACCg--GAu-- -3' miRNA: 3'- gaCGa-CCGGACCAUGUGGaaaCUucc -5' |
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| 19216 | 5' | -52.3 | NC_004684.1 | + | 1997 | 0.75 | 0.431978 |
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Target: 5'- aCUGCUGGCCUGG-GCGCUggaccgccccgacgUUGAccAGGc -3' miRNA: 3'- -GACGACCGGACCaUGUGGa-------------AACU--UCC- -5' |
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| 19216 | 5' | -52.3 | NC_004684.1 | + | 36121 | 0.77 | 0.330006 |
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Target: 5'- cCUGCUGGCCUucgccACACCUgUGggGGc -3' miRNA: 3'- -GACGACCGGAcca--UGUGGAaACuuCC- -5' |
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| 19216 | 5' | -52.3 | NC_004684.1 | + | 41233 | 1.1 | 0.001999 |
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Target: 5'- gCUGCUGGCCUGGUACACCUUUGAAGGc -3' miRNA: 3'- -GACGACCGGACCAUGUGGAAACUUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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