miRNA: 3'- -CGGa--------CCGGGACGAGU------------AGGUgGAGAAG- -5'
miRNA: 3'- -CGG-------------ACCGGgACGAGUAGGUGGAgaag -5'
miRNA: 3'- -CGGACCGGGACGAGUAGGUGGAGAAG- -5'
Results 21 - 23 of 23 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 19226 | 5' | -57.5 | NC_004684.1 | + | 13407 | 0.75 | 0.19417 |
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Target: 5'- aGCCggacgugucGGCCCUGCUCAccgacuucgcguUCCAgUUCUUCu -3' miRNA: 3'- -CGGa--------CCGGGACGAGU------------AGGUgGAGAAG- -5' |
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| 19226 | 5' | -57.5 | NC_004684.1 | + | 23477 | 0.77 | 0.136565 |
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Target: 5'- cGCCaucaaccaggagaUGGCCgUGCUCAUCCACCUg--- -3' miRNA: 3'- -CGG-------------ACCGGgACGAGUAGGUGGAgaag -5' |
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| 19226 | 5' | -57.5 | NC_004684.1 | + | 45516 | 1.12 | 0.000435 |
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Target: 5'- gGCCUGGCCCUGCUCAUCCACCUCUUCg -3' miRNA: 3'- -CGGACCGGGACGAGUAGGUGGAGAAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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