Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1923 | 5' | -66 | NC_001347.2 | + | 199323 | 0.66 | 0.551986 |
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Target: 5'- gGCC-GGCAUGGGUaugcauucguugagGCGGCGGaGGu -3' miRNA: 3'- aCGGcCCGUACCCGg-------------CGCCGCCaCCu -5' |
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| 1923 | 5' | -66 | NC_001347.2 | + | 36 | 0.66 | 0.566697 |
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Target: 5'- gUGUuuUGGGUGUgucGGGgCGCGGCcgGGUGGGu -3' miRNA: 3'- -ACG--GCCCGUA---CCCgGCGCCG--CCACCU- -5' |
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| 1923 | 5' | -66 | NC_001347.2 | + | 78348 | 0.66 | 0.566697 |
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Target: 5'- gGuCCGGGCggacccggcgugGUGGGaCCcgGCGGCgccguGGUGGGa -3' miRNA: 3'- aC-GGCCCG------------UACCC-GG--CGCCG-----CCACCU- -5' |
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| 1923 | 5' | -66 | NC_001347.2 | + | 44031 | 0.66 | 0.575942 |
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Target: 5'- cGCgGuGGCGacGGCaGCGGUGGUGGc -3' miRNA: 3'- aCGgC-CCGUacCCGgCGCCGCCACCu -5' |
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| 1923 | 5' | -66 | NC_001347.2 | + | 140670 | 0.66 | 0.585221 |
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Target: 5'- -uCCGGGgGaaugaUGGGgCGCGGCGGUcgcauguuGGGc -3' miRNA: 3'- acGGCCCgU-----ACCCgGCGCCGCCA--------CCU- -5' |
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| 1923 | 5' | -66 | NC_001347.2 | + | 209736 | 0.66 | 0.585221 |
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Target: 5'- cUGCCGGaGCAccgUGGGUgcCGGCGGUa-- -3' miRNA: 3'- -ACGGCC-CGU---ACCCGgcGCCGCCAccu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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