Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 1923 | 5' | -66 | NC_001347.2 | + | 1630 | 0.67 | 0.477077 |
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Target: 5'- gGCUGGGCGUGGGCacccCGCGcGCGa---- -3' miRNA: 3'- aCGGCCCGUACCCG----GCGC-CGCcaccu -5' |
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| 1923 | 5' | -66 | NC_001347.2 | + | 75404 | 0.67 | 0.485748 |
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Target: 5'- aGCgGGaGCGgcGGCCGUGGCGGcGGc -3' miRNA: 3'- aCGgCC-CGUacCCGGCGCCGCCaCCu -5' |
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| 1923 | 5' | -66 | NC_001347.2 | + | 106218 | 0.67 | 0.485748 |
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Target: 5'- cGCCGGcCAcGagccagacgaGGCgGCGGCGGUGGc -3' miRNA: 3'- aCGGCCcGUaC----------CCGgCGCCGCCACCu -5' |
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| 1923 | 5' | -66 | NC_001347.2 | + | 82693 | 0.67 | 0.503308 |
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Target: 5'- aGCUggucccgaacaGGGUGUacGGGUgGUGGCGGUGGu -3' miRNA: 3'- aCGG-----------CCCGUA--CCCGgCGCCGCCACCu -5' |
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| 1923 | 5' | -66 | NC_001347.2 | + | 184961 | 0.67 | 0.485748 |
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Target: 5'- gGCgUGGGCGgcucgcggagacUGGGCUGCaGGCGGcagcGGAu -3' miRNA: 3'- aCG-GCCCGU------------ACCCGGCG-CCGCCa---CCU- -5' |
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| 1923 | 5' | -66 | NC_001347.2 | + | 140670 | 0.66 | 0.585221 |
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Target: 5'- -uCCGGGgGaaugaUGGGgCGCGGCGGUcgcauguuGGGc -3' miRNA: 3'- acGGCCCgU-----ACCCgGCGCCGCCA--------CCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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