Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 19244 | 3' | -53.5 | NC_004684.1 | + | 56691 | 0.71 | 0.574964 |
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Target: 5'- uGCcgGUUgcgagccaccgcgUCCCGACcguugaggcguucGGugUGCCGGUCg -3' miRNA: 3'- -CGuaCAG-------------AGGGCUG-------------UUugACGGCCAG- -5' |
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| 19244 | 3' | -53.5 | NC_004684.1 | + | 6919 | 0.71 | 0.559798 |
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Target: 5'- -gGUGUUgCCCGAgGAACUGCCcggcaugcuggccgaGGUCg -3' miRNA: 3'- cgUACAGaGGGCUgUUUGACGG---------------CCAG- -5' |
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| 19244 | 3' | -53.5 | NC_004684.1 | + | 49529 | 0.71 | 0.555484 |
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Target: 5'- aGCGUG-CaCCCGGCGGugUGCgCGGUg -3' miRNA: 3'- -CGUACaGaGGGCUGUUugACG-GCCAg -5' |
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| 19244 | 3' | -53.5 | NC_004684.1 | + | 9306 | 0.72 | 0.502534 |
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Target: 5'- gGCGcG-CUCCCGGuCGAGCUGCaCGGUg -3' miRNA: 3'- -CGUaCaGAGGGCU-GUUUGACG-GCCAg -5' |
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| 19244 | 3' | -53.5 | NC_004684.1 | + | 44553 | 0.72 | 0.491172 |
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Target: 5'- ---gGUCUuggccagCCCGGCcugcaccgccggGAACUGCCGGUCg -3' miRNA: 3'- cguaCAGA-------GGGCUG------------UUUGACGGCCAG- -5' |
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| 19244 | 3' | -53.5 | NC_004684.1 | + | 60582 | 0.73 | 0.461814 |
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Target: 5'- gGUAUGUCUCCaCGccgcGCAcgguGCgcagGCCGGUCa -3' miRNA: 3'- -CGUACAGAGG-GC----UGUu---UGa---CGGCCAG- -5' |
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| 19244 | 3' | -53.5 | NC_004684.1 | + | 57454 | 1.11 | 0.001352 |
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Target: 5'- cGCAUGUCUCCCGACAAACUGCCGGUCc -3' miRNA: 3'- -CGUACAGAGGGCUGUUUGACGGCCAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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