Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 19253 | 5' | -54.2 | NC_004684.1 | + | 67194 | 0.68 | 0.727524 |
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Target: 5'- cGGGuCACGGuCUCGCCGuUCGGcagGUCa- -3' miRNA: 3'- -CCU-GUGCCuGAGUGGC-AGCUa--CAGgu -5' |
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| 19253 | 5' | -54.2 | NC_004684.1 | + | 49223 | 0.69 | 0.696024 |
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Target: 5'- uGGACACcGACgCGCCGauuUCGAUGUugCCGa -3' miRNA: 3'- -CCUGUGcCUGaGUGGC---AGCUACA--GGU- -5' |
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| 19253 | 5' | -54.2 | NC_004684.1 | + | 57692 | 0.69 | 0.685392 |
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Target: 5'- aGGACAC---CUCGCCGUCGAUGguuuuggUCAg -3' miRNA: 3'- -CCUGUGccuGAGUGGCAGCUACa------GGU- -5' |
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| 19253 | 5' | -54.2 | NC_004684.1 | + | 37191 | 0.69 | 0.674711 |
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Target: 5'- --cCAUGGACUCGCCGgguUUGGUGgCCAc -3' miRNA: 3'- ccuGUGCCUGAGUGGC---AGCUACaGGU- -5' |
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| 19253 | 5' | -54.2 | NC_004684.1 | + | 46765 | 0.7 | 0.631699 |
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Target: 5'- cGGACGCaGGCggcgcaggaACCGUCGAccGUCCGg -3' miRNA: 3'- -CCUGUGcCUGag-------UGGCAGCUa-CAGGU- -5' |
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| 19253 | 5' | -54.2 | NC_004684.1 | + | 43936 | 0.7 | 0.620923 |
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Target: 5'- cGGGCAUuggcucccaGGuCUCGCCGUCGAgg-CCGa -3' miRNA: 3'- -CCUGUG---------CCuGAGUGGCAGCUacaGGU- -5' |
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| 19253 | 5' | -54.2 | NC_004684.1 | + | 63839 | 0.7 | 0.610157 |
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Target: 5'- uGGCGCGGcGCaCGCUGUCGGUG-CCAc -3' miRNA: 3'- cCUGUGCC-UGaGUGGCAGCUACaGGU- -5' |
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| 19253 | 5' | -54.2 | NC_004684.1 | + | 18459 | 0.72 | 0.494646 |
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Target: 5'- aGGACgACGGcaccgaGCUggUGCUGUCGGUGUCCGg -3' miRNA: 3'- -CCUG-UGCC------UGA--GUGGCAGCUACAGGU- -5' |
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| 19253 | 5' | -54.2 | NC_004684.1 | + | 60830 | 1.11 | 0.001495 |
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Target: 5'- cGGACACGGACUCACCGUCGAUGUCCAc -3' miRNA: 3'- -CCUGUGCCUGAGUGGCAGCUACAGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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