Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 19255 | 3' | -55.7 | NC_004684.1 | + | 49193 | 0.7 | 0.536854 |
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Target: 5'- gCGGCgGcGGccUGCUCGGUCGGCCAg- -3' miRNA: 3'- -GCUGaC-CCacAUGAGCCAGUCGGUgg -5' |
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| 19255 | 3' | -55.7 | NC_004684.1 | + | 11060 | 0.7 | 0.536854 |
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Target: 5'- cCGuCUGGaGgacggcaagGUGCUCGGUUcgGGCUACCu -3' miRNA: 3'- -GCuGACC-Ca--------CAUGAGCCAG--UCGGUGG- -5' |
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| 19255 | 3' | -55.7 | NC_004684.1 | + | 36784 | 0.71 | 0.475923 |
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Target: 5'- cCGACgcuGGUcGgcggcgGCUCGGUCAGCUACg -3' miRNA: 3'- -GCUGac-CCA-Ca-----UGAGCCAGUCGGUGg -5' |
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| 19255 | 3' | -55.7 | NC_004684.1 | + | 66027 | 0.72 | 0.41863 |
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Target: 5'- uCGGCcaGGGUGUugGCgUUGGUCAGCCagACCc -3' miRNA: 3'- -GCUGa-CCCACA--UG-AGCCAGUCGG--UGG- -5' |
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| 19255 | 3' | -55.7 | NC_004684.1 | + | 52431 | 0.72 | 0.41863 |
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Target: 5'- aGGCcGGGgGU-CUCGauGUCGGCCGCCa -3' miRNA: 3'- gCUGaCCCaCAuGAGC--CAGUCGGUGG- -5' |
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| 19255 | 3' | -55.7 | NC_004684.1 | + | 25712 | 0.73 | 0.349149 |
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Target: 5'- cCGcGCUGGGUGuUGCcgaGGUCGGCCcgGCCu -3' miRNA: 3'- -GC-UGACCCAC-AUGag-CCAGUCGG--UGG- -5' |
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| 19255 | 3' | -55.7 | NC_004684.1 | + | 51017 | 0.76 | 0.242147 |
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Target: 5'- uCGAUgugccGGGUGUACUCGGccgccagcuugUCGGCCAgCg -3' miRNA: 3'- -GCUGa----CCCACAUGAGCC-----------AGUCGGUgG- -5' |
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| 19255 | 3' | -55.7 | NC_004684.1 | + | 37390 | 0.85 | 0.066069 |
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Target: 5'- -cGCUGGGUGUGCUCGG-CAGCUugCc -3' miRNA: 3'- gcUGACCCACAUGAGCCaGUCGGugG- -5' |
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| 19255 | 3' | -55.7 | NC_004684.1 | + | 61148 | 1.11 | 0.000966 |
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Target: 5'- gCGACUGGGUGUACUCGGUCAGCCACCc -3' miRNA: 3'- -GCUGACCCACAUGAGCCAGUCGGUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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