Results 81 - 86 of 86 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 19256 | 5' | -63.1 | NC_004684.1 | + | 23212 | 0.66 | 0.381872 |
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Target: 5'- gGCCGCCGGUGgcgacggcggguucCUgaaauggcucCGCGacGCCACCa- -3' miRNA: 3'- gCGGCGGCCAC--------------GA----------GCGCa-CGGUGGau -5' |
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| 19256 | 5' | -63.1 | NC_004684.1 | + | 62736 | 0.66 | 0.384397 |
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Target: 5'- uCGUCGUgGGUGCUgGgaCGUGCCAUg-- -3' miRNA: 3'- -GCGGCGgCCACGAgC--GCACGGUGgau -5' |
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| 19256 | 5' | -63.1 | NC_004684.1 | + | 16266 | 0.66 | 0.384397 |
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Target: 5'- -uUCGUCGGcagaUGCUCGCG-GCCauGCCUGc -3' miRNA: 3'- gcGGCGGCC----ACGAGCGCaCGG--UGGAU- -5' |
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| 19256 | 5' | -63.1 | NC_004684.1 | + | 9665 | 0.66 | 0.384397 |
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Target: 5'- uGCCGCCuGg----GCGUGCCACCa- -3' miRNA: 3'- gCGGCGGcCacgagCGCACGGUGGau -5' |
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| 19256 | 5' | -63.1 | NC_004684.1 | + | 62490 | 0.66 | 0.384397 |
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Target: 5'- uGCCGCCGaGgccGgUgGCGaccagGCCGCCUGa -3' miRNA: 3'- gCGGCGGC-Ca--CgAgCGCa----CGGUGGAU- -5' |
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| 19256 | 5' | -63.1 | NC_004684.1 | + | 14487 | 0.66 | 0.38778 |
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Target: 5'- aCGCCGCC-GUGCUgGCcgGacgcuccugggugcaUGCCACCg- -3' miRNA: 3'- -GCGGCGGcCACGAgCG--C---------------ACGGUGGau -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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