Results 81 - 86 of 86 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 19256 | 5' | -63.1 | NC_004684.1 | + | 4294 | 0.67 | 0.295056 |
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Target: 5'- uGCCGCCGGgucgccguaauguagUGCUUGCacggcggcacgGUaGCCGCCa- -3' miRNA: 3'- gCGGCGGCC---------------ACGAGCG-----------CA-CGGUGGau -5' |
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| 19256 | 5' | -63.1 | NC_004684.1 | + | 4035 | 0.69 | 0.229457 |
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Target: 5'- gGCCGCuCGGUGCgcuggGCG-GUCACCg- -3' miRNA: 3'- gCGGCG-GCCACGag---CGCaCGGUGGau -5' |
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| 19256 | 5' | -63.1 | NC_004684.1 | + | 2449 | 0.66 | 0.351656 |
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Target: 5'- uCGcCCGCCuGGgcggccaGCUCgGCGUGCCguaccACCUGa -3' miRNA: 3'- -GC-GGCGG-CCa------CGAG-CGCACGG-----UGGAU- -5' |
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| 19256 | 5' | -63.1 | NC_004684.1 | + | 2352 | 0.66 | 0.351656 |
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Target: 5'- uGCCGCCcugaGUUCGUG-GCCGCCa- -3' miRNA: 3'- gCGGCGGcca-CGAGCGCaCGGUGGau -5' |
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| 19256 | 5' | -63.1 | NC_004684.1 | + | 1571 | 0.76 | 0.068641 |
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Target: 5'- aGCCGCCGGUGcCUC-CGgGUCACCUGc -3' miRNA: 3'- gCGGCGGCCAC-GAGcGCaCGGUGGAU- -5' |
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| 19256 | 5' | -63.1 | NC_004684.1 | + | 748 | 0.71 | 0.178426 |
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Target: 5'- cCGCCGCCGaG-GCcaUCGUG-GCCACCa- -3' miRNA: 3'- -GCGGCGGC-CaCG--AGCGCaCGGUGGau -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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