Results 81 - 86 of 86 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 19256 | 5' | -63.1 | NC_004684.1 | + | 22853 | 0.68 | 0.265662 |
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Target: 5'- uGCCGaCCGG-GUUCGUccggGCCACCa- -3' miRNA: 3'- gCGGC-GGCCaCGAGCGca--CGGUGGau -5' |
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| 19256 | 5' | -63.1 | NC_004684.1 | + | 51611 | 0.69 | 0.241039 |
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Target: 5'- aCGuuGCCGGUG-UCGCcgccgcgauagGUGCCGCUg- -3' miRNA: 3'- -GCggCGGCCACgAGCG-----------CACGGUGGau -5' |
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| 19256 | 5' | -63.1 | NC_004684.1 | + | 40544 | 0.69 | 0.229457 |
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Target: 5'- uGCuCGCUGGUGCUCGaccgGuCCACCa- -3' miRNA: 3'- gCG-GCGGCCACGAGCgca-C-GGUGGau -5' |
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| 19256 | 5' | -63.1 | NC_004684.1 | + | 4035 | 0.69 | 0.229457 |
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Target: 5'- gGCCGCuCGGUGCgcuggGCG-GUCACCg- -3' miRNA: 3'- gCGGCG-GCCACGag---CGCaCGGUGGau -5' |
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| 19256 | 5' | -63.1 | NC_004684.1 | + | 40796 | 0.69 | 0.223844 |
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Target: 5'- cCGCCGCCGGUgGCgaaccCGCGcacaCCGCCg- -3' miRNA: 3'- -GCGGCGGCCA-CGa----GCGCac--GGUGGau -5' |
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| 19256 | 5' | -63.1 | NC_004684.1 | + | 48972 | 0.79 | 0.041873 |
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Target: 5'- gGCCGCCGGUGCgccCGCcggGCCGCCg- -3' miRNA: 3'- gCGGCGGCCACGa--GCGca-CGGUGGau -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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