Results 81 - 86 of 86 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 19256 | 5' | -63.1 | NC_004684.1 | + | 53535 | 0.67 | 0.306356 |
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Target: 5'- uGUCGUCGGUGCg-GCGUGCCu---- -3' miRNA: 3'- gCGGCGGCCACGagCGCACGGuggau -5' |
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| 19256 | 5' | -63.1 | NC_004684.1 | + | 56806 | 0.67 | 0.313585 |
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Target: 5'- uGCCGUCGGUGaCggaCGUG-GCCAUCg- -3' miRNA: 3'- gCGGCGGCCAC-Ga--GCGCaCGGUGGau -5' |
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| 19256 | 5' | -63.1 | NC_004684.1 | + | 58164 | 0.67 | 0.313585 |
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Target: 5'- gGgaGCCGGUGCgacUGUGCCGCCa- -3' miRNA: 3'- gCggCGGCCACGagcGCACGGUGGau -5' |
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| 19256 | 5' | -63.1 | NC_004684.1 | + | 26436 | 0.67 | 0.320943 |
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Target: 5'- uCGCCGCCGGUgGCcCG-GUGUacggcggcaCGCCUGg -3' miRNA: 3'- -GCGGCGGCCA-CGaGCgCACG---------GUGGAU- -5' |
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| 19256 | 5' | -63.1 | NC_004684.1 | + | 9548 | 0.67 | 0.327675 |
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Target: 5'- gCGCCgguuggaGCCGGUGCgcCGCGaacacgugGCCgACCUGa -3' miRNA: 3'- -GCGG-------CGGCCACGa-GCGCa-------CGG-UGGAU- -5' |
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| 19256 | 5' | -63.1 | NC_004684.1 | + | 23212 | 0.66 | 0.381872 |
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Target: 5'- gGCCGCCGGUGgcgacggcggguucCUgaaauggcucCGCGacGCCACCa- -3' miRNA: 3'- gCGGCGGCCAC--------------GA----------GCGCa-CGGUGGau -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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