Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 19259 | 3' | -51.4 | NC_004684.1 | + | 60171 | 0.66 | 0.93225 |
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Target: 5'- --cGGUgGCAG-UGUaGgGGAGCGGGu -3' miRNA: 3'- ugaCCAgCGUCuACAaCaUCUCGUCC- -5' |
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| 19259 | 3' | -51.4 | NC_004684.1 | + | 15302 | 0.66 | 0.93225 |
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Target: 5'- cCUGGUCGaCGuggccGGUGgcaUGGAGCGGGc -3' miRNA: 3'- uGACCAGC-GU-----CUACaacAUCUCGUCC- -5' |
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| 19259 | 3' | -51.4 | NC_004684.1 | + | 64844 | 0.66 | 0.914311 |
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Target: 5'- cUUGGUagGCGGGUgGUUGUGGuGgGGGg -3' miRNA: 3'- uGACCAg-CGUCUA-CAACAUCuCgUCC- -5' |
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| 19259 | 3' | -51.4 | NC_004684.1 | + | 41934 | 0.66 | 0.914311 |
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Target: 5'- --aGGUCGCuGAUGgcaccGGAGCAGu -3' miRNA: 3'- ugaCCAGCGuCUACaaca-UCUCGUCc -5' |
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| 19259 | 3' | -51.4 | NC_004684.1 | + | 55820 | 0.66 | 0.913023 |
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Target: 5'- gGCUGGuguggaacUCGCGGuagccgcugugGUUGcGGGGCGGGu -3' miRNA: 3'- -UGACC--------AGCGUCua---------CAACaUCUCGUCC- -5' |
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| 19259 | 3' | -51.4 | NC_004684.1 | + | 31442 | 0.66 | 0.905062 |
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Target: 5'- cGCUG-UCGUAGAacagccgcccGUAGAGCAGGu -3' miRNA: 3'- -UGACcAGCGUCUacaa------CAUCUCGUCC- -5' |
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| 19259 | 3' | -51.4 | NC_004684.1 | + | 56290 | 0.68 | 0.854274 |
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Target: 5'- cCUGGUCgGCGugcucGUUGUuGAGCAGGu -3' miRNA: 3'- uGACCAG-CGUcua--CAACAuCUCGUCC- -5' |
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| 19259 | 3' | -51.4 | NC_004684.1 | + | 9189 | 0.69 | 0.779043 |
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Target: 5'- -aUGGaCGUAGGUGUUGUAcuuguccAGCAGGu -3' miRNA: 3'- ugACCaGCGUCUACAACAUc------UCGUCC- -5' |
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| 19259 | 3' | -51.4 | NC_004684.1 | + | 64461 | 1.1 | 0.002526 |
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Target: 5'- gACUGGUCGCAGAUGUUGUAGAGCAGGc -3' miRNA: 3'- -UGACCAGCGUCUACAACAUCUCGUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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