Results 1 - 14 of 14 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
19263 | 3' | -56.4 | NC_004685.1 | + | 43798 | 0.66 | 0.724035 |
Target: 5'- cGGAGucgaucaGCGUCggccGCCCCGAccuGCGCGg -3' miRNA: 3'- -UCUCug-----CGCAGa---UGGGGUUcu-CGCGU- -5' |
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19263 | 3' | -56.4 | NC_004685.1 | + | 19851 | 0.66 | 0.703 |
Target: 5'- cGAGAUGaUGUCcacCCCCGAG-GUGCGg -3' miRNA: 3'- uCUCUGC-GCAGau-GGGGUUCuCGCGU- -5' |
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19263 | 3' | -56.4 | NC_004685.1 | + | 33052 | 0.66 | 0.69982 |
Target: 5'- -cGGugGCGUUgacggcgaugagcuUGCCCgCGGcGAGCGCAc -3' miRNA: 3'- ucUCugCGCAG--------------AUGGG-GUU-CUCGCGU- -5' |
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19263 | 3' | -56.4 | NC_004685.1 | + | 7646 | 0.66 | 0.692378 |
Target: 5'- uGAuGACGCGgugcgCUggaugaacgcgcAUCCCGAGAGUGCc -3' miRNA: 3'- uCU-CUGCGCa----GA------------UGGGGUUCUCGCGu -5' |
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19263 | 3' | -56.4 | NC_004685.1 | + | 14526 | 0.67 | 0.670975 |
Target: 5'- cGAuGcCGCaUCUGcguacCCCCGAGGGCGCGc -3' miRNA: 3'- uCU-CuGCGcAGAU-----GGGGUUCUCGCGU- -5' |
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19263 | 3' | -56.4 | NC_004685.1 | + | 8274 | 0.67 | 0.660216 |
Target: 5'- aAGGGugGCGgggcguUCUGCCCCugaAGCGgGa -3' miRNA: 3'- -UCUCugCGC------AGAUGGGGuucUCGCgU- -5' |
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19263 | 3' | -56.4 | NC_004685.1 | + | 17338 | 0.67 | 0.617024 |
Target: 5'- -aGGugGCGaCUACUUCAAGcgGGCGCGg -3' miRNA: 3'- ucUCugCGCaGAUGGGGUUC--UCGCGU- -5' |
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19263 | 3' | -56.4 | NC_004685.1 | + | 60040 | 0.68 | 0.595467 |
Target: 5'- gGGGGugGCGUC-GCCCUucGGGGUGg- -3' miRNA: 3'- -UCUCugCGCAGaUGGGGu-UCUCGCgu -5' |
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19263 | 3' | -56.4 | NC_004685.1 | + | 28681 | 0.68 | 0.552777 |
Target: 5'- --cGAuCGCGUCggcgacGCCaCCAGGAGUGCGc -3' miRNA: 3'- ucuCU-GCGCAGa-----UGG-GGUUCUCGCGU- -5' |
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19263 | 3' | -56.4 | NC_004685.1 | + | 57691 | 0.71 | 0.432011 |
Target: 5'- cGAGA-GCaUCUGCCCCAcgucGAGCGCc -3' miRNA: 3'- uCUCUgCGcAGAUGGGGUu---CUCGCGu -5' |
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19263 | 3' | -56.4 | NC_004685.1 | + | 45950 | 0.73 | 0.328083 |
Target: 5'- cGGAGuAgGCGUCUuccaGCCCCAGuGGCGCu -3' miRNA: 3'- -UCUC-UgCGCAGA----UGGGGUUcUCGCGu -5' |
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19263 | 3' | -56.4 | NC_004685.1 | + | 10620 | 0.76 | 0.203523 |
Target: 5'- cGGAGGCGCGggcCUACaaccugaagcugCCCAAGGGUGCGa -3' miRNA: 3'- -UCUCUGCGCa--GAUG------------GGGUUCUCGCGU- -5' |
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19263 | 3' | -56.4 | NC_004685.1 | + | 2401 | 0.77 | 0.188088 |
Target: 5'- --cGACGCG-CUGCCCgCAAGcAGCGCAa -3' miRNA: 3'- ucuCUGCGCaGAUGGG-GUUC-UCGCGU- -5' |
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19263 | 3' | -56.4 | NC_004685.1 | + | 69558 | 1.09 | 0.001022 |
Target: 5'- gAGAGACGCGUCUACCCCAAGAGCGCAg -3' miRNA: 3'- -UCUCUGCGCAGAUGGGGUUCUCGCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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