Results 1 - 20 of 22 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
19266 | 5' | -63.1 | NC_004685.1 | + | 64083 | 0.66 | 0.376101 |
Target: 5'- --cCCCCGCGU---CCCGCAGGuCUGg -3' miRNA: 3'- uacGGGGCGUAggcGGGCGUCCuGAC- -5' |
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19266 | 5' | -63.1 | NC_004685.1 | + | 43731 | 0.66 | 0.376101 |
Target: 5'- -gGCCgCGaCAUUCcCCCGCucGGACUGg -3' miRNA: 3'- uaCGGgGC-GUAGGcGGGCGu-CCUGAC- -5' |
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19266 | 5' | -63.1 | NC_004685.1 | + | 21072 | 0.66 | 0.367814 |
Target: 5'- -cGUCCgGcCGUCgGUCUGCAGGGCg- -3' miRNA: 3'- uaCGGGgC-GUAGgCGGGCGUCCUGac -5' |
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19266 | 5' | -63.1 | NC_004685.1 | + | 62294 | 0.66 | 0.367814 |
Target: 5'- -cGCCCuCGCA-CCGCuuGCcgucGGGACc- -3' miRNA: 3'- uaCGGG-GCGUaGGCGggCG----UCCUGac -5' |
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19266 | 5' | -63.1 | NC_004685.1 | + | 49651 | 0.66 | 0.343717 |
Target: 5'- --aCCCCGCGaugaUCCGCCCcgGCGGGuCg- -3' miRNA: 3'- uacGGGGCGU----AGGCGGG--CGUCCuGac -5' |
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19266 | 5' | -63.1 | NC_004685.1 | + | 67981 | 0.66 | 0.343717 |
Target: 5'- --aCCCCGCAUUCGCCgaGUggaugauGGGCUGg -3' miRNA: 3'- uacGGGGCGUAGGCGGg-CGu------CCUGAC- -5' |
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19266 | 5' | -63.1 | NC_004685.1 | + | 12861 | 0.67 | 0.311926 |
Target: 5'- uUGCCgCCGCAgcucucgccgcccaaUCCGCUgcugcccucgucgaGCGGGGCUGa -3' miRNA: 3'- uACGG-GGCGU---------------AGGCGGg-------------CGUCCUGAC- -5' |
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19266 | 5' | -63.1 | NC_004685.1 | + | 2235 | 0.67 | 0.299002 |
Target: 5'- gGUGCcagaCCUGCcugCCGCCCGgCcGGGCUGa -3' miRNA: 3'- -UACG----GGGCGua-GGCGGGC-GuCCUGAC- -5' |
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19266 | 5' | -63.1 | NC_004685.1 | + | 24540 | 0.67 | 0.298296 |
Target: 5'- cGUGUCCCGCAUCCccgucgacgagacGCCCuGCGaaguGaGGCUGg -3' miRNA: 3'- -UACGGGGCGUAGG-------------CGGG-CGU----C-CUGAC- -5' |
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19266 | 5' | -63.1 | NC_004685.1 | + | 36775 | 0.67 | 0.291309 |
Target: 5'- -aGCCCUucgacugGCGgcCCGagCCGCAGGGCUGg -3' miRNA: 3'- uaCGGGG-------CGUa-GGCg-GGCGUCCUGAC- -5' |
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19266 | 5' | -63.1 | NC_004685.1 | + | 6817 | 0.67 | 0.285131 |
Target: 5'- -gGCUacuUCGCA-CCGCCCGCAGGcGCa- -3' miRNA: 3'- uaCGG---GGCGUaGGCGGGCGUCC-UGac -5' |
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19266 | 5' | -63.1 | NC_004685.1 | + | 66840 | 0.67 | 0.285131 |
Target: 5'- -cGCCCUGCAcguuggCCgGCCCGCAGaGCg- -3' miRNA: 3'- uaCGGGGCGUa-----GG-CGGGCGUCcUGac -5' |
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19266 | 5' | -63.1 | NC_004685.1 | + | 37537 | 0.68 | 0.278389 |
Target: 5'- gGUGCCCUGCGUCggcaCGCCCaGC-GGugUc -3' miRNA: 3'- -UACGGGGCGUAG----GCGGG-CGuCCugAc -5' |
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19266 | 5' | -63.1 | NC_004685.1 | + | 31761 | 0.68 | 0.265286 |
Target: 5'- -aGCUCCGaccacCCGCCCGCuGGGgUGg -3' miRNA: 3'- uaCGGGGCgua--GGCGGGCGuCCUgAC- -5' |
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19266 | 5' | -63.1 | NC_004685.1 | + | 49357 | 0.69 | 0.240585 |
Target: 5'- -cGCCgCCGcCGUCCgGCCCGUcGGGCa- -3' miRNA: 3'- uaCGG-GGC-GUAGG-CGGGCGuCCUGac -5' |
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19266 | 5' | -63.1 | NC_004685.1 | + | 1126 | 0.69 | 0.223343 |
Target: 5'- --aCCCCgacGCAUCggggCGCCCGCAucGGACUGg -3' miRNA: 3'- uacGGGG---CGUAG----GCGGGCGU--CCUGAC- -5' |
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19266 | 5' | -63.1 | NC_004685.1 | + | 68496 | 0.69 | 0.215662 |
Target: 5'- -gGCCCCGCcaguccgaugcggggCCGCCaguccgaUGCGGGGCUGu -3' miRNA: 3'- uaCGGGGCGua-------------GGCGG-------GCGUCCUGAC- -5' |
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19266 | 5' | -63.1 | NC_004685.1 | + | 14553 | 0.7 | 0.182448 |
Target: 5'- -aGCCCCGaCcgCCGCCacuGCuGGAUUGg -3' miRNA: 3'- uaCGGGGC-GuaGGCGGg--CGuCCUGAC- -5' |
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19266 | 5' | -63.1 | NC_004685.1 | + | 206 | 0.7 | 0.177829 |
Target: 5'- cAUGCCCCGCGUCaucaaaggcgacUGCCCGCGuuuGACg- -3' miRNA: 3'- -UACGGGGCGUAG------------GCGGGCGUc--CUGac -5' |
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19266 | 5' | -63.1 | NC_004685.1 | + | 1170 | 0.77 | 0.064462 |
Target: 5'- -aGCCCCGCAUCgGacuggcggcCCCGCAucGGACUGg -3' miRNA: 3'- uaCGGGGCGUAGgC---------GGGCGU--CCUGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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