Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 19267 | 5' | -54.4 | NC_004685.1 | + | 35429 | 0.66 | 0.853347 |
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Target: 5'- aCUCGGCGGuGcGUCaGGUGgucgaCGUGGc -3' miRNA: 3'- gGAGCUGCCuCaCAGaCUACg----GCACC- -5' |
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| 19267 | 5' | -54.4 | NC_004685.1 | + | 7155 | 0.67 | 0.818977 |
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Target: 5'- gUUCGGCGGgguggccaucgcGGUGUCcGA-GCCGUcGGg -3' miRNA: 3'- gGAGCUGCC------------UCACAGaCUaCGGCA-CC- -5' |
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| 19267 | 5' | -54.4 | NC_004685.1 | + | 13990 | 0.67 | 0.809901 |
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Target: 5'- ---gGACGGAGUG-CUGcAUGuCCGUGc -3' miRNA: 3'- ggagCUGCCUCACaGAC-UAC-GGCACc -5' |
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| 19267 | 5' | -54.4 | NC_004685.1 | + | 790 | 0.68 | 0.731793 |
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Target: 5'- uCCUCGGCGGuGaUGacUCcGuugGCCGUGGu -3' miRNA: 3'- -GGAGCUGCCuC-AC--AGaCua-CGGCACC- -5' |
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| 19267 | 5' | -54.4 | NC_004685.1 | + | 53515 | 0.76 | 0.329707 |
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Target: 5'- uCCUCGugGGGGUGguacaucgaggcUUUGcgGUCGUGGc -3' miRNA: 3'- -GGAGCugCCUCAC------------AGACuaCGGCACC- -5' |
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| 19267 | 5' | -54.4 | NC_004685.1 | + | 68391 | 1.11 | 0.00147 |
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Target: 5'- uCCUCGACGGAGUGUCUGAUGCCGUGGc -3' miRNA: 3'- -GGAGCUGCCUCACAGACUACGGCACC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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