Results 1 - 14 of 14 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
19268 | 3' | -50.2 | NC_004685.1 | + | 67180 | 0.66 | 0.961962 |
Target: 5'- --cGCGGccuccUUGCGCCCGGUCgGCcagcaCGAg -3' miRNA: 3'- cuuUGCU-----AACGCGGGUUAGaCGa----GCU- -5' |
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19268 | 3' | -50.2 | NC_004685.1 | + | 3855 | 0.66 | 0.949273 |
Target: 5'- cGAggUGGgccagUGCcuGCUCGcgCUGCUCGGg -3' miRNA: 3'- -CUuuGCUa----ACG--CGGGUuaGACGAGCU- -5' |
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19268 | 3' | -50.2 | NC_004685.1 | + | 55346 | 0.67 | 0.922441 |
Target: 5'- -cGACGGguugGCGUUCAuccaCUGCUCGAu -3' miRNA: 3'- cuUUGCUaa--CGCGGGUua--GACGAGCU- -5' |
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19268 | 3' | -50.2 | NC_004685.1 | + | 22863 | 0.68 | 0.909674 |
Target: 5'- cGAggUGucgGCGCCCGG-CUGC-CGAc -3' miRNA: 3'- -CUuuGCuaaCGCGGGUUaGACGaGCU- -5' |
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19268 | 3' | -50.2 | NC_004685.1 | + | 22336 | 0.68 | 0.902858 |
Target: 5'- ---cUGcgUGCGCUCGcgCUGUUCGGc -3' miRNA: 3'- cuuuGCuaACGCGGGUuaGACGAGCU- -5' |
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19268 | 3' | -50.2 | NC_004685.1 | + | 19232 | 0.68 | 0.895756 |
Target: 5'- --uGCGGUUcGCGCCgAGUCUGCcCa- -3' miRNA: 3'- cuuUGCUAA-CGCGGgUUAGACGaGcu -5' |
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19268 | 3' | -50.2 | NC_004685.1 | + | 51448 | 0.68 | 0.888375 |
Target: 5'- --uGCGAgcaccgUGCGCCCAGcCgacgacaGCUCGAu -3' miRNA: 3'- cuuUGCUa-----ACGCGGGUUaGa------CGAGCU- -5' |
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19268 | 3' | -50.2 | NC_004685.1 | + | 32373 | 0.68 | 0.880718 |
Target: 5'- cGAGGCcc-UGCGCCCAcugcuccaaCUGCUCGGc -3' miRNA: 3'- -CUUUGcuaACGCGGGUua-------GACGAGCU- -5' |
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19268 | 3' | -50.2 | NC_004685.1 | + | 38311 | 0.7 | 0.819995 |
Target: 5'- aGGACGGUgaagGCGCgggccuggCCGAUCUGCgCGAg -3' miRNA: 3'- cUUUGCUAa---CGCG--------GGUUAGACGaGCU- -5' |
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19268 | 3' | -50.2 | NC_004685.1 | + | 38400 | 0.71 | 0.759747 |
Target: 5'- --cGCGGUgGCGUCCucguUCUGCUCGu -3' miRNA: 3'- cuuUGCUAaCGCGGGuu--AGACGAGCu -5' |
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19268 | 3' | -50.2 | NC_004685.1 | + | 35170 | 0.72 | 0.727601 |
Target: 5'- --uACGuggUGCGCaCCAAcccgCUGCUCGAu -3' miRNA: 3'- cuuUGCua-ACGCG-GGUUa---GACGAGCU- -5' |
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19268 | 3' | -50.2 | NC_004685.1 | + | 37110 | 0.72 | 0.71337 |
Target: 5'- --uGCGGUucggauccauguucUGCGCCgCGAUCUGCUCc- -3' miRNA: 3'- cuuUGCUA--------------ACGCGG-GUUAGACGAGcu -5' |
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19268 | 3' | -50.2 | NC_004685.1 | + | 36698 | 0.8 | 0.30855 |
Target: 5'- --uGCG-UUGCGCCCu-UCUGCUCGAu -3' miRNA: 3'- cuuUGCuAACGCGGGuuAGACGAGCU- -5' |
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19268 | 3' | -50.2 | NC_004685.1 | + | 68251 | 0.94 | 0.043315 |
Target: 5'- cGAAACGAUguCGCCCAAUCUGCUCGAg -3' miRNA: 3'- -CUUUGCUAacGCGGGUUAGACGAGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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