Results 1 - 20 of 29 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
19271 | 3' | -61.3 | NC_004685.1 | + | 45739 | 0.66 | 0.475623 |
Target: 5'- aCGGcagcggGUCGCCggGGCGGCG-CGUg -3' miRNA: 3'- -GCCaua---CGGCGGgaCCGCCGCuGCAa -5' |
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19271 | 3' | -61.3 | NC_004685.1 | + | 29070 | 0.66 | 0.466037 |
Target: 5'- ---cGUGCUGaCCgCUGGCgcGGCGGCGUUc -3' miRNA: 3'- gccaUACGGC-GG-GACCG--CCGCUGCAA- -5' |
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19271 | 3' | -61.3 | NC_004685.1 | + | 61941 | 0.66 | 0.466037 |
Target: 5'- gCGGUcGUGCaCGUCCUGGCuGCGGg--- -3' miRNA: 3'- -GCCA-UACG-GCGGGACCGcCGCUgcaa -5' |
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19271 | 3' | -61.3 | NC_004685.1 | + | 53819 | 0.66 | 0.466037 |
Target: 5'- gGGUGUGuaGCCCUccugaaggucGGcCGGUGGCGc- -3' miRNA: 3'- gCCAUACggCGGGA----------CC-GCCGCUGCaa -5' |
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19271 | 3' | -61.3 | NC_004685.1 | + | 40126 | 0.66 | 0.456552 |
Target: 5'- gCGGaaguUGUCGgUCaUGGCGGCGAUGUc -3' miRNA: 3'- -GCCau--ACGGCgGG-ACCGCCGCUGCAa -5' |
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19271 | 3' | -61.3 | NC_004685.1 | + | 59461 | 0.66 | 0.447172 |
Target: 5'- aCGGUcaGCuCGCCgUuggacagcucgGGCGGCGGCGg- -3' miRNA: 3'- -GCCAuaCG-GCGGgA-----------CCGCCGCUGCaa -5' |
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19271 | 3' | -61.3 | NC_004685.1 | + | 402 | 0.66 | 0.447172 |
Target: 5'- -----aGCUGCUCguaGGCGGCGGCGa- -3' miRNA: 3'- gccauaCGGCGGGa--CCGCCGCUGCaa -5' |
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19271 | 3' | -61.3 | NC_004685.1 | + | 37973 | 0.66 | 0.4379 |
Target: 5'- aGGcGUGgaugagauucgcCCGCgCCUGGUGGUGACGc- -3' miRNA: 3'- gCCaUAC------------GGCG-GGACCGCCGCUGCaa -5' |
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19271 | 3' | -61.3 | NC_004685.1 | + | 42821 | 0.67 | 0.410762 |
Target: 5'- cCGGccagGUCGCCCUGuuugcgcugaGCGGCGGCa-- -3' miRNA: 3'- -GCCaua-CGGCGGGAC----------CGCCGCUGcaa -5' |
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19271 | 3' | -61.3 | NC_004685.1 | + | 59562 | 0.67 | 0.401951 |
Target: 5'- gGGUcgcagcAUGuuGCgCaGGCGGCGGCGg- -3' miRNA: 3'- gCCA------UACggCGgGaCCGCCGCUGCaa -5' |
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19271 | 3' | -61.3 | NC_004685.1 | + | 64182 | 0.67 | 0.37625 |
Target: 5'- -----gGCCGCUC-GGCGGCGAUGg- -3' miRNA: 3'- gccauaCGGCGGGaCCGCCGCUGCaa -5' |
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19271 | 3' | -61.3 | NC_004685.1 | + | 17676 | 0.68 | 0.367933 |
Target: 5'- cCGGg--GCCGCCCcGGCGuCGGCa-- -3' miRNA: 3'- -GCCauaCGGCGGGaCCGCcGCUGcaa -5' |
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19271 | 3' | -61.3 | NC_004685.1 | + | 53587 | 0.68 | 0.367933 |
Target: 5'- uCGGcgGUGuuGCCCUGGUaGCGaAUGUg -3' miRNA: 3'- -GCCa-UACggCGGGACCGcCGC-UGCAa -5' |
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19271 | 3' | -61.3 | NC_004685.1 | + | 26952 | 0.68 | 0.359744 |
Target: 5'- gGGUGUGCaCGUCgCggaacgaGGUGGCGGCGa- -3' miRNA: 3'- gCCAUACG-GCGG-Ga------CCGCCGCUGCaa -5' |
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19271 | 3' | -61.3 | NC_004685.1 | + | 32751 | 0.68 | 0.359744 |
Target: 5'- uGGUGgaccgGCCGCCagguUGGUGGCGAa--- -3' miRNA: 3'- gCCAUa----CGGCGGg---ACCGCCGCUgcaa -5' |
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19271 | 3' | -61.3 | NC_004685.1 | + | 62624 | 0.68 | 0.351682 |
Target: 5'- aGGUcgGCUgucgugucgGCCCUGGCGuugcgcagcGCGGCGa- -3' miRNA: 3'- gCCAuaCGG---------CGGGACCGC---------CGCUGCaa -5' |
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19271 | 3' | -61.3 | NC_004685.1 | + | 39214 | 0.68 | 0.351682 |
Target: 5'- -----cGCCG-CCUGGCGGCGGCu-- -3' miRNA: 3'- gccauaCGGCgGGACCGCCGCUGcaa -5' |
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19271 | 3' | -61.3 | NC_004685.1 | + | 36397 | 0.68 | 0.326754 |
Target: 5'- uGGgcaacuuccCCGCCCUGGUGcGCGGCGa- -3' miRNA: 3'- gCCauac-----GGCGGGACCGC-CGCUGCaa -5' |
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19271 | 3' | -61.3 | NC_004685.1 | + | 37517 | 0.69 | 0.32073 |
Target: 5'- aGGUGUgGCgCGCUgUGGUGGCcGCGUUc -3' miRNA: 3'- gCCAUA-CG-GCGGgACCGCCGcUGCAA- -5' |
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19271 | 3' | -61.3 | NC_004685.1 | + | 53221 | 0.69 | 0.319983 |
Target: 5'- aGGaUGUGCCGCaCCaucagguagguggUGGCGGCcuGGCGUc -3' miRNA: 3'- gCC-AUACGGCG-GG-------------ACCGCCG--CUGCAa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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