Results 1 - 16 of 16 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
19273 | 3' | -57.3 | NC_004685.1 | + | 32634 | 0.66 | 0.691985 |
Target: 5'- ---gUGGGUGGCGgaagCGGUgGUGGUGAa -3' miRNA: 3'- accgACCCACUGCaa--GCCG-CGCUACU- -5' |
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19273 | 3' | -57.3 | NC_004685.1 | + | 62260 | 0.66 | 0.670835 |
Target: 5'- gGGCggGGaGUGAuaCGcgCGGCGCGcUGGc -3' miRNA: 3'- aCCGa-CC-CACU--GCaaGCCGCGCuACU- -5' |
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19273 | 3' | -57.3 | NC_004685.1 | + | 33180 | 0.66 | 0.660204 |
Target: 5'- cGGCcgGuGGUgGGCGc-CGGUGCGGUGGa -3' miRNA: 3'- aCCGa-C-CCA-CUGCaaGCCGCGCUACU- -5' |
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19273 | 3' | -57.3 | NC_004685.1 | + | 46671 | 0.66 | 0.659139 |
Target: 5'- gUGGC-GGGUGGCaUcccaccgggccuaUCGGCGgCGAUGc -3' miRNA: 3'- -ACCGaCCCACUGcA-------------AGCCGC-GCUACu -5' |
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19273 | 3' | -57.3 | NC_004685.1 | + | 7362 | 0.67 | 0.617528 |
Target: 5'- aGGCgUGGGUGA---UCGGUGCccacGAUGGu -3' miRNA: 3'- aCCG-ACCCACUgcaAGCCGCG----CUACU- -5' |
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19273 | 3' | -57.3 | NC_004685.1 | + | 45604 | 0.67 | 0.596225 |
Target: 5'- gGGgUGGGUGcUGUUCGuCGCGAUc- -3' miRNA: 3'- aCCgACCCACuGCAAGCcGCGCUAcu -5' |
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19273 | 3' | -57.3 | NC_004685.1 | + | 35936 | 0.68 | 0.564507 |
Target: 5'- cGGCUGGGUgucggcgaucguGACGUUCcgcaacGGCGaGGUGu -3' miRNA: 3'- aCCGACCCA------------CUGCAAG------CCGCgCUACu -5' |
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19273 | 3' | -57.3 | NC_004685.1 | + | 15268 | 0.68 | 0.561357 |
Target: 5'- gGGCUGucgccgaucgagaaGGUGAUGaUCGGUGaGAUGAc -3' miRNA: 3'- aCCGAC--------------CCACUGCaAGCCGCgCUACU- -5' |
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19273 | 3' | -57.3 | NC_004685.1 | + | 15181 | 0.68 | 0.554026 |
Target: 5'- aGGUUGGGcacaucugccaUGACGaggCGGCauuacGCGGUGAg -3' miRNA: 3'- aCCGACCC-----------ACUGCaa-GCCG-----CGCUACU- -5' |
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19273 | 3' | -57.3 | NC_004685.1 | + | 45744 | 0.68 | 0.522963 |
Target: 5'- gGGCUGGGgGugGUc---CGCGAUGAg -3' miRNA: 3'- aCCGACCCaCugCAagccGCGCUACU- -5' |
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19273 | 3' | -57.3 | NC_004685.1 | + | 15156 | 0.69 | 0.512755 |
Target: 5'- gGuGCUGGGcuUGACGcUgGGUGCGAUcGAg -3' miRNA: 3'- aC-CGACCC--ACUGCaAgCCGCGCUA-CU- -5' |
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19273 | 3' | -57.3 | NC_004685.1 | + | 43039 | 0.7 | 0.453423 |
Target: 5'- cGGCUGuGGUGugcuCG-UCGGUGCGgcGGu -3' miRNA: 3'- aCCGAC-CCACu---GCaAGCCGCGCuaCU- -5' |
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19273 | 3' | -57.3 | NC_004685.1 | + | 47588 | 0.71 | 0.371999 |
Target: 5'- uUGGUggucGGUG-CGUUCGGUGCgGAUGAc -3' miRNA: 3'- -ACCGac--CCACuGCAAGCCGCG-CUACU- -5' |
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19273 | 3' | -57.3 | NC_004685.1 | + | 21599 | 0.72 | 0.315922 |
Target: 5'- cGGCUGGGUcggcACGUUCGGUGCc---- -3' miRNA: 3'- aCCGACCCAc---UGCAAGCCGCGcuacu -5' |
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19273 | 3' | -57.3 | NC_004685.1 | + | 41756 | 0.74 | 0.266463 |
Target: 5'- aGGCaucGGUGGCGUUgaCGGCGCGGUcGAg -3' miRNA: 3'- aCCGac-CCACUGCAA--GCCGCGCUA-CU- -5' |
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19273 | 3' | -57.3 | NC_004685.1 | + | 66537 | 1.09 | 0.000856 |
Target: 5'- aUGGCUGGGUGACGUUCGGCGCGAUGAg -3' miRNA: 3'- -ACCGACCCACUGCAAGCCGCGCUACU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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