Results 1 - 20 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
19273 | 5' | -58.9 | NC_004685.1 | + | 66571 | 1.08 | 0.000636 |
Target: 5'- cGGCCUCACCCAGGACGUCACCCUGAAa -3' miRNA: 3'- -CCGGAGUGGGUCCUGCAGUGGGACUU- -5' |
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19273 | 5' | -58.9 | NC_004685.1 | + | 50424 | 0.74 | 0.206738 |
Target: 5'- gGGCCUCAUCCAcGACGUCgcagaacACCUUGGg -3' miRNA: 3'- -CCGGAGUGGGUcCUGCAG-------UGGGACUu -5' |
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19273 | 5' | -58.9 | NC_004685.1 | + | 61666 | 0.72 | 0.253783 |
Target: 5'- aGCCcgUCGCCCAGGuACGUCgcGCCCaGAAc -3' miRNA: 3'- cCGG--AGUGGGUCC-UGCAG--UGGGaCUU- -5' |
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19273 | 5' | -58.9 | NC_004685.1 | + | 6689 | 0.72 | 0.260166 |
Target: 5'- gGGCCaaggaGCCCgAGGugGgcggCACCCUGAc -3' miRNA: 3'- -CCGGag---UGGG-UCCugCa---GUGGGACUu -5' |
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19273 | 5' | -58.9 | NC_004685.1 | + | 51983 | 0.72 | 0.266679 |
Target: 5'- cGCCaa--CCAGGGCgGUCACCCUGAu -3' miRNA: 3'- cCGGagugGGUCCUG-CAGUGGGACUu -5' |
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19273 | 5' | -58.9 | NC_004685.1 | + | 62900 | 0.71 | 0.287009 |
Target: 5'- uGGCUUCGaCCAGGGCGUCACgCaGAc -3' miRNA: 3'- -CCGGAGUgGGUCCUGCAGUGgGaCUu -5' |
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19273 | 5' | -58.9 | NC_004685.1 | + | 16362 | 0.69 | 0.371863 |
Target: 5'- aGUCgaugagCGCCCAGGACcUCAgCCUGAu -3' miRNA: 3'- cCGGa-----GUGGGUCCUGcAGUgGGACUu -5' |
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19273 | 5' | -58.9 | NC_004685.1 | + | 9973 | 0.69 | 0.389017 |
Target: 5'- uGGCCgacgaCGCCguGGGCGagaugcUCACCCUGu- -3' miRNA: 3'- -CCGGa----GUGGguCCUGC------AGUGGGACuu -5' |
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19273 | 5' | -58.9 | NC_004685.1 | + | 9041 | 0.69 | 0.415702 |
Target: 5'- cGGUCgUCACCCuGGACGggucgcgCACCCg--- -3' miRNA: 3'- -CCGG-AGUGGGuCCUGCa------GUGGGacuu -5' |
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19273 | 5' | -58.9 | NC_004685.1 | + | 42774 | 0.68 | 0.423923 |
Target: 5'- cGCCUacaacuaCACCCAGGAucCGUCggugucguggACCCUGGc -3' miRNA: 3'- cCGGA-------GUGGGUCCU--GCAG----------UGGGACUu -5' |
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19273 | 5' | -58.9 | NC_004685.1 | + | 63021 | 0.68 | 0.462565 |
Target: 5'- cGGCCUUGCgCAGcGCGUCACCgaUGGc -3' miRNA: 3'- -CCGGAGUGgGUCcUGCAGUGGg-ACUu -5' |
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19273 | 5' | -58.9 | NC_004685.1 | + | 51284 | 0.67 | 0.501974 |
Target: 5'- -cCCUCGCUguGcGAgGUCACCUUGAu -3' miRNA: 3'- ccGGAGUGGguC-CUgCAGUGGGACUu -5' |
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19273 | 5' | -58.9 | NC_004685.1 | + | 46349 | 0.67 | 0.501974 |
Target: 5'- cGGCCUCAgacUCCAGGACcUCaggcucaucaACCUUGGg -3' miRNA: 3'- -CCGGAGU---GGGUCCUGcAG----------UGGGACUu -5' |
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19273 | 5' | -58.9 | NC_004685.1 | + | 65931 | 0.67 | 0.522225 |
Target: 5'- aGCUUCGgCCGGGcgaacACGUCGCCCa--- -3' miRNA: 3'- cCGGAGUgGGUCC-----UGCAGUGGGacuu -5' |
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19273 | 5' | -58.9 | NC_004685.1 | + | 39481 | 0.67 | 0.522225 |
Target: 5'- uGGCCgcggCGCCCAGGcCGacgagCGCgCUGGu -3' miRNA: 3'- -CCGGa---GUGGGUCCuGCa----GUGgGACUu -5' |
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19273 | 5' | -58.9 | NC_004685.1 | + | 29108 | 0.66 | 0.553164 |
Target: 5'- cGGCCgcgCcgGCCCAGGugGUCGacaCCg--- -3' miRNA: 3'- -CCGGa--G--UGGGUCCugCAGUg--GGacuu -5' |
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19273 | 5' | -58.9 | NC_004685.1 | + | 66406 | 0.66 | 0.563602 |
Target: 5'- cGGCC-CGacggcaCCAGGAUGUUGCCCa--- -3' miRNA: 3'- -CCGGaGUg-----GGUCCUGCAGUGGGacuu -5' |
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19273 | 5' | -58.9 | NC_004685.1 | + | 61495 | 0.66 | 0.563602 |
Target: 5'- cGGCCgacCGCCCGGcGGCGUCGCg----- -3' miRNA: 3'- -CCGGa--GUGGGUC-CUGCAGUGggacuu -5' |
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19273 | 5' | -58.9 | NC_004685.1 | + | 65854 | 0.66 | 0.563602 |
Target: 5'- cGCCgcgUACuCCAGGGCGUCccgucGCCCgacGAGa -3' miRNA: 3'- cCGGa--GUG-GGUCCUGCAG-----UGGGa--CUU- -5' |
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19273 | 5' | -58.9 | NC_004685.1 | + | 48070 | 0.66 | 0.584627 |
Target: 5'- cGGCUUCGCgCAGGAUGgCACCg---- -3' miRNA: 3'- -CCGGAGUGgGUCCUGCaGUGGgacuu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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