Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
19273 | 5' | -58.9 | NC_004685.1 | + | 6689 | 0.72 | 0.260166 |
Target: 5'- gGGCCaaggaGCCCgAGGugGgcggCACCCUGAc -3' miRNA: 3'- -CCGGag---UGGG-UCCugCa---GUGGGACUu -5' |
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19273 | 5' | -58.9 | NC_004685.1 | + | 61666 | 0.72 | 0.253783 |
Target: 5'- aGCCcgUCGCCCAGGuACGUCgcGCCCaGAAc -3' miRNA: 3'- cCGG--AGUGGGUCC-UGCAG--UGGGaCUU- -5' |
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19273 | 5' | -58.9 | NC_004685.1 | + | 50424 | 0.74 | 0.206738 |
Target: 5'- gGGCCUCAUCCAcGACGUCgcagaacACCUUGGg -3' miRNA: 3'- -CCGGAGUGGGUcCUGCAG-------UGGGACUu -5' |
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19273 | 5' | -58.9 | NC_004685.1 | + | 66571 | 1.08 | 0.000636 |
Target: 5'- cGGCCUCACCCAGGACGUCACCCUGAAa -3' miRNA: 3'- -CCGGAGUGGGUCCUGCAGUGGGACUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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