Results 1 - 19 of 19 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
19275 | 3' | -57.7 | NC_004685.1 | + | 8411 | 0.66 | 0.644382 |
Target: 5'- uGCcuGcGGCUGCCCgaCGGUGUGgUCgGCa -3' miRNA: 3'- -UGuuC-CCGACGGGa-GUCACGU-AGgCG- -5' |
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19275 | 3' | -57.7 | NC_004685.1 | + | 57250 | 0.66 | 0.644382 |
Target: 5'- gGCGcAGGGCUGCCgccuuCUCGGcgaugGC--CCGCa -3' miRNA: 3'- -UGU-UCCCGACGG-----GAGUCa----CGuaGGCG- -5' |
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19275 | 3' | -57.7 | NC_004685.1 | + | 20339 | 0.66 | 0.644382 |
Target: 5'- cCGAGGacgacacacuGCUGCCgUCGGagcUGCuggCCGCg -3' miRNA: 3'- uGUUCC----------CGACGGgAGUC---ACGua-GGCG- -5' |
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19275 | 3' | -57.7 | NC_004685.1 | + | 20167 | 0.66 | 0.644382 |
Target: 5'- uGCAGGGGUcgaauacggcUGCCCcgaGGUGg--CCGCa -3' miRNA: 3'- -UGUUCCCG----------ACGGGag-UCACguaGGCG- -5' |
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19275 | 3' | -57.7 | NC_004685.1 | + | 3471 | 0.66 | 0.622819 |
Target: 5'- -uGAGGGCaGCCCUUGuugGCGUcguggCCGCa -3' miRNA: 3'- ugUUCCCGaCGGGAGUca-CGUA-----GGCG- -5' |
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19275 | 3' | -57.7 | NC_004685.1 | + | 10715 | 0.66 | 0.60774 |
Target: 5'- cACGGGaGGCUGCCauggagcauuggCAGcGCGUgCGCg -3' miRNA: 3'- -UGUUC-CCGACGGga----------GUCaCGUAgGCG- -5' |
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19275 | 3' | -57.7 | NC_004685.1 | + | 39449 | 0.66 | 0.601288 |
Target: 5'- gGCAAGGGCcGCgCCUgGGUcCAguUCCGg -3' miRNA: 3'- -UGUUCCCGaCG-GGAgUCAcGU--AGGCg -5' |
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19275 | 3' | -57.7 | NC_004685.1 | + | 68478 | 0.66 | 0.601288 |
Target: 5'- cCAAGGGUugUGCCgCgcagUGGUGCGacUCUGCa -3' miRNA: 3'- uGUUCCCG--ACGG-Ga---GUCACGU--AGGCG- -5' |
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19275 | 3' | -57.7 | NC_004685.1 | + | 2549 | 0.66 | 0.601288 |
Target: 5'- -gAAGGuGCgUGCCCUCcaGGcUGCAcugcgCCGCg -3' miRNA: 3'- ugUUCC-CG-ACGGGAG--UC-ACGUa----GGCG- -5' |
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19275 | 3' | -57.7 | NC_004685.1 | + | 1151 | 0.66 | 0.590556 |
Target: 5'- gACcAGGGUgGCCCUCGgccaucGUGCAgCgGCa -3' miRNA: 3'- -UGuUCCCGaCGGGAGU------CACGUaGgCG- -5' |
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19275 | 3' | -57.7 | NC_004685.1 | + | 37316 | 0.66 | 0.590556 |
Target: 5'- aGCAGuGGGCgcagGgCCUCGGUGCcgagAUCauCGCg -3' miRNA: 3'- -UGUU-CCCGa---CgGGAGUCACG----UAG--GCG- -5' |
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19275 | 3' | -57.7 | NC_004685.1 | + | 31229 | 0.67 | 0.558593 |
Target: 5'- gGCAAGGGCgucggcucggGCCUggUCGGcaucGCggCCGCg -3' miRNA: 3'- -UGUUCCCGa---------CGGG--AGUCa---CGuaGGCG- -5' |
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19275 | 3' | -57.7 | NC_004685.1 | + | 66065 | 0.68 | 0.506536 |
Target: 5'- gGCAAgcGGGCgGUCgUUGGUGCcgCCGUa -3' miRNA: 3'- -UGUU--CCCGaCGGgAGUCACGuaGGCG- -5' |
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19275 | 3' | -57.7 | NC_004685.1 | + | 15081 | 0.69 | 0.437456 |
Target: 5'- ---cGGGgaGCUgCUCGGUGCcgagGUCCGCu -3' miRNA: 3'- uguuCCCgaCGG-GAGUCACG----UAGGCG- -5' |
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19275 | 3' | -57.7 | NC_004685.1 | + | 21652 | 0.71 | 0.348924 |
Target: 5'- uGCAGGuGGUcgaGCCUUCGGUGUucgGUCUGCa -3' miRNA: 3'- -UGUUC-CCGa--CGGGAGUCACG---UAGGCG- -5' |
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19275 | 3' | -57.7 | NC_004685.1 | + | 64414 | 0.71 | 0.340803 |
Target: 5'- aAC-AGGGCUGCCa-UAGUGC-UCUGCc -3' miRNA: 3'- -UGuUCCCGACGGgaGUCACGuAGGCG- -5' |
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19275 | 3' | -57.7 | NC_004685.1 | + | 60706 | 0.71 | 0.324975 |
Target: 5'- cACAcccGGCUGaCCCUCGGcGCccAUCCGCa -3' miRNA: 3'- -UGUuc-CCGAC-GGGAGUCaCG--UAGGCG- -5' |
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19275 | 3' | -57.7 | NC_004685.1 | + | 39548 | 0.73 | 0.267182 |
Target: 5'- gGCcGGGGCUGCCgggCcgAGUGCggCCGCg -3' miRNA: 3'- -UGuUCCCGACGGga-G--UCACGuaGGCG- -5' |
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19275 | 3' | -57.7 | NC_004685.1 | + | 66222 | 1.11 | 0.000438 |
Target: 5'- aACAAGGGCUGCCCUCAGUGCAUCCGCg -3' miRNA: 3'- -UGUUCCCGACGGGAGUCACGUAGGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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