Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 19276 | 5' | -55.2 | NC_004685.1 | + | 39527 | 0.66 | 0.777899 |
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Target: 5'- cGGUCCCGCgauACCUG-CGAuggccgGGGCUGc-- -3' miRNA: 3'- -CUAGGGUG---UGGACuGCU------CCCGAUaca -5' |
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| 19276 | 5' | -55.2 | NC_004685.1 | + | 16319 | 0.66 | 0.767898 |
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Target: 5'- cAUCCCGCGCUggUGACGGGcGGUg---- -3' miRNA: 3'- cUAGGGUGUGG--ACUGCUC-CCGauaca -5' |
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| 19276 | 5' | -55.2 | NC_004685.1 | + | 43466 | 0.66 | 0.767898 |
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Target: 5'- -cUCgCGCGCCUGAaguccggcguCGGGGGCggUAUGa -3' miRNA: 3'- cuAGgGUGUGGACU----------GCUCCCG--AUACa -5' |
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| 19276 | 5' | -55.2 | NC_004685.1 | + | 65265 | 0.67 | 0.726626 |
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Target: 5'- --aCCCACACCcuUGuCGGGGG-UGUGg -3' miRNA: 3'- cuaGGGUGUGG--ACuGCUCCCgAUACa -5' |
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| 19276 | 5' | -55.2 | NC_004685.1 | + | 34977 | 0.67 | 0.673027 |
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Target: 5'- -cUCCCACGCCaucuacauCGGcGGGCUGUGc -3' miRNA: 3'- cuAGGGUGUGGacu-----GCU-CCCGAUACa -5' |
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| 19276 | 5' | -55.2 | NC_004685.1 | + | 37499 | 0.68 | 0.651245 |
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Target: 5'- --aCCCGCccagcCCUGGCGAGGuguggcgcGCUGUGg -3' miRNA: 3'- cuaGGGUGu----GGACUGCUCC--------CGAUACa -5' |
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| 19276 | 5' | -55.2 | NC_004685.1 | + | 31833 | 0.69 | 0.585792 |
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Target: 5'- cGAUCCCGCugCc-GC-AGGGCUAUGc -3' miRNA: 3'- -CUAGGGUGugGacUGcUCCCGAUACa -5' |
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| 19276 | 5' | -55.2 | NC_004685.1 | + | 11273 | 0.69 | 0.574966 |
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Target: 5'- uGAUCCCGCugaACCgGACGAuGGGCg---- -3' miRNA: 3'- -CUAGGGUG---UGGaCUGCU-CCCGauaca -5' |
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| 19276 | 5' | -55.2 | NC_004685.1 | + | 13846 | 0.7 | 0.547053 |
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Target: 5'- --aCCCACAcCCUGACcgcggugcuguucGGGCUGUGUg -3' miRNA: 3'- cuaGGGUGU-GGACUGcu-----------CCCGAUACA- -5' |
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| 19276 | 5' | -55.2 | NC_004685.1 | + | 65569 | 1.07 | 0.001718 |
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Target: 5'- cGAUCCCACACCUGACGAGGGCUAUGUc -3' miRNA: 3'- -CUAGGGUGUGGACUGCUCCCGAUACA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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